A Lin28 homologue reprograms differentiated cells to stem cells in the moss Physcomitrella patens.
A Lin28 homologue reprograms differentiated cells to stem cells in the moss Physcomitrella patens.
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DOI：
10.1038/ncomms14242
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发表时间：
2017-01-27
影响因子：
16.6
通讯作者：
Hasebe M
中科院分区：
文献类型：
--
作者：
Li C;Sako Y;Imai A;Nishiyama T;Thompson K;Kubo M;Hiwatashi Y;Kabeya Y;Karlson D;Wu SH;Ishikawa M;Murata T;Benfey PN;Sato Y;Tamada Y;Hasebe M
陆地植物和后生动物都有能力将分化的细胞重新编程为干细胞。在这里，我们表明，苔藓小立碗藓冷休克结构域蛋白1（PpCSP 1）调节重编程分化叶细胞绿线顶端干细胞和股份保守的结构域与哺乳动物中的诱导多能干细胞因子林28。PpCSP 1在重编程细胞中积累，并在整个重编程过程和所得干细胞中维持。PpCSP 1的表达受其3′-非翻译区（3′-UTR）负调控。去除3′-UTR稳定PpCSP 1转录物，导致PpCSP 1蛋白积累并增强重编程。PpCSP 1和三个密切相关的PpCSP基因的四重缺失突变体表现出减弱的重编程，表明PpCSP基因在细胞重编程中冗余地发挥作用。综上所述，这些数据证明了PpCSP 1在重编程中的积极作用，这与哺乳动物Lin 28的功能相似。陆地植物和后生动物都能够在某些情况下将分化的细胞重新编程为干细胞。在这里，作者表明，苔藓CSP 1蛋白，与哺乳动物多能干细胞因子Lin 28共享保守结构域，促进叶细胞重编程为顶端干细胞。
Both land plants and metazoa have the capacity to reprogram differentiated cells to stem cells. Here we show that the moss Physcomitrella patens Cold-Shock Domain Protein 1 (PpCSP1) regulates reprogramming of differentiated leaf cells to chloronema apical stem cells and shares conserved domains with the induced pluripotent stem cell factor Lin28 in mammals. PpCSP1 accumulates in the reprogramming cells and is maintained throughout the reprogramming process and in the resultant stem cells. Expression of PpCSP1 is negatively regulated by its 3′-untranslated region (3′-UTR). Removal of the 3′-UTR stabilizes PpCSP1 transcripts, results in accumulation of PpCSP1 protein and enhances reprogramming. A quadruple deletion mutant of PpCSP1 and three closely related PpCSP genes exhibits attenuated reprogramming indicating that the PpCSP genes function redundantly in cellular reprogramming. Taken together, these data demonstrate a positive role of PpCSP1 in reprogramming, which is similar to the function of mammalian Lin28. Land plants and metazoans are both able to reprogram differentiated cells to stem cells under certain circumstances. Here the authors show that the moss CSP1 protein, which shares conserved domains with the mammalian pluripotent stem cell factor Lin28, promotes reprogramming of leaf cells to apical stem cells.