Fluorescence turn-on sensing of ascorbic acid and alkaline phosphatase activity based on graphene quantum dots
Fluorescence turn-on sensing of ascorbic acid and alkaline phosphatase activity based on graphene quantum dots
复制标题
基于石墨烯量子点的抗坏血酸和碱性磷酸酶活性的荧光开启传感
DOI：
10.1016/j.snb.2016.05.080
复制
发表时间：
2016-11-01
影响因子：
8.4
通讯作者：
Liu, Zhenning
中科院分区：
文献类型：
--
作者：
Huang, Hui;Wang, Bingdi;Liu, Zhenning
A facile fluorescence turn-on approach for the detection of L-ascorbic acid (AA) and alkaline phosphatase (ALP) activity was designed based on graphene quantum dots (GQDs). It is found that the fluorescence intensity of GQDs can be efficiently quenched by para-benzoquinone (BQ). And a turn-on fluorescence signal can be observed if BQis reduced by AA. In addition, ALP can hydrolyze L-ascorbic acid-2-phosphate (AAP) to produce AA, which also results in the recovery of the GQDs fluorescence. The recovered fluorescence intensity of GQDs was proportional to the concentration of AA or ALP in the concentration ranges of 5.0 x 10(-7) to 2.0 x 10(-5) mol/L and 1.0-90 U/L, respectively. The detection limit for AA and ALP were 2.0 x 10(-7) mol/L and 0.45 U/L. The established method showed a high selectivity for ALP activity compared with other interference components. Furthermore, the detection system as a fluorescence probe has great potential in the development of sensors for widely detecting various analytes with AA producing process. (C) 2016 Elsevier B.V. All rights reserved.
