Label-free electrochemical detection of DNA in blood serum via target-induced resolution of an electrode-bound DNA pseudoknot
Label-free electrochemical detection of DNA in blood serum via target-induced resolution of an electrode-bound DNA pseudoknot
复制标题
DOI：
10.1021/ja074218y
复制
发表时间：
2007-10-03
影响因子：
15
通讯作者：
Heeger, Alan J.
中科院分区：
文献类型：
--
作者：
Xiao, Yi;Qu, Xiaogang;Heeger, Alan J.
我们已经证明了一个完全共价的，信号上的E-DNA架构的基础上的目标诱导的分辨率的DNA pseudokont。在不存在靶的情况下，电极结合的DNA探针采用假结构象，其将附着的亚甲蓝（MB）与电极分离。靶结合后，假结被分解，导致形成单链DNA元件，其支持从亚甲蓝到电极的电子转移。
We have demonstrated a fully covalent, signal-on E-DNA architecture based on the target-induced resolution of a DNA pseudokont. In the absence of target, the electrode-bound DNA probe adopts a pseudoknot conformation that segregates an attached methylene blue (MB) from the electrode. Upon target binding, the pseudoknot is resolved, leading to the formation of a single-stranded DNA element that supports electron transfer from the methylene blue to the electrode.