Identification of galactose-α-1,3-galactose in the gastrointestinal tract of the tick Ixode sricinus; possible relationship with red meat allergy
Identification of galactose-α-1,3-galactose in the gastrointestinal tract of the tick Ixode sricinus; possible relationship with red meat allergy
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DOI：
10.1111/all.12128
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发表时间：
2013-04-01
期刊：
影响因子：
12.4
通讯作者：
van Hage, M.
中科院分区：
文献类型：
--
作者：
Hamsten, C.;Starkhammar, M.;van Hage, M.
患有针对碳水化合物表位半乳糖--1，3-半乳糖（-Gal）的IgE抗体的患者在食用红肉后报告了严重的过敏反应。调查显示IgE对-Gal和蜱叮咬之间的关联。我们提供了第一个直接的证据，-半乳糖是存在于蜱，从而可能解释蜱暴露和致敏性之间的关系-半乳糖，与发展的红肉过敏作为次要现象。来自瑞典的对红肉有迟发性严重反应的患者的血清被纳入研究。证明了蜱Ixodesricinus对-Gal的IgE反应的剂量依赖性抑制。此外，使用cryostat切割部分的I.蓖麻，我们表明，单克隆抗体和多克隆抗体对-Gal染色的胃肠道的蜱。当用对-Gal呈阳性的患者血清IgE染色时，观察到相同的模式。这些结果证实了-Gal表位存在于I.ricinus中，并意味着宿主在蜱叮咬期间暴露于-Gal。这提供了进一步的证据表明，蜱叮咬与IgE反应-半乳糖和红肉过敏。
Patients with IgE antibodies against the carbohydrate epitope galactose--1,3-galactose (-Gal) have reported severe allergic reactions after consumption of red meat. Investigations have revealed associations between IgE to -Gal and tick bites. We provide the first direct evidence that -Gal is present within ticks thus potentially explaining the relationship between tick exposure and sensitization to -Gal, with development of red meat allergy as a secondary phenomena. Serum from Swedish patients with delayed severe reactions to red meat was included in the study. A dose-dependent inhibition of IgE responses to -Gal by the tick Ixodesricinus is demonstrated. Furthermore, using cryostat-cut sections of I.ricinus, we show that both a monoclonal and a polyclonal antibody against -Gal stains the gastrointestinal tract of the tick. The same pattern is seen when staining with patient sera IgE positive to -Gal. These results confirm that the -Gal epitope is present in I.ricinus and imply host exposure to -Gal during a tick bite. This provides further evidence that tick bites are associated with IgE responses to -Gal and red meat allergy.