Discovery of a new antifungal lipopeptaibol from Purpureocillium lilacinum using MALDI-TOF-IMS
Discovery of a new antifungal lipopeptaibol from Purpureocillium lilacinum using MALDI-TOF-IMS
复制标题
使用 MALDI-TOF-IMS 从紫紫紫霉中发现新型抗真菌脂肽醇
DOI：
10.1016/j.bbrc.2020.05.021
复制
发表时间：
2020
影响因子：
3.1
通讯作者：
Xie Bingyan
中科院分区：
文献类型：
--
作者：
Liu Rui;Khan Raja Asad Ali;Yue Qun;Jiao Yang;Yang Yuhong;Li Yan;Xie Bingyan
真菌被认为富含用于农业和医药目的的生物活性天然产物。发现和准确鉴定具有生物活性的真菌天然产物对有效利用真菌天然产物具有重要意义。在我们不断寻找新的真菌天然产物的过程中，我们发现了著名的生防真菌淡紫紫孢菌对灰葡萄孢的体外活性，灰葡萄孢是一种气传植物病原真菌，引起许多蔬菜和水果的灰霉病。两种真菌在琼脂平板上共培养的结果表明，淡紫青霉对灰霉病菌的生长有抑制作用，说明淡紫青霉具有产生抗灰霉病菌活性次生代谢产物的潜力。本研究应用基质辅助激光解吸电离飞行时间质谱成像质谱（MALDI-TOF-IMS）技术，从淡紫青霉菌中发现一种新的抗真菌脂肽素（leucinostatin Z）。通过LC-HRESI-MS-MS分析进一步确定了Leucinostatin Z的平面结构。MALDI-TOF-IMS是一种新的方法，它可以直接在两种真菌菌落之间的生长培养基上观察生物活性天然产物，比传统技术更快，更有效地发现真菌中新的生物活性化合物。
Fungi are considered to be rich in biologically active natural products for agricultural and medicinal purposes. The discovery and accurate identification of the bioactive fungal natural products is important for their efficient utilization. During the course of our continuing search for the new natural products from the fungal agents, we found the well-known bio-control fungusPurpureocillium lilacinumshowedin vitroactivity againstBotrytis cinerea, an airborne plant pathogenic fungus causing gray mold disease in many vegetables and fruits. The co-culture of two fungi on agar plate showed thatP. lilacinuminhibited the growth ofB. cinereawhich meansP. lilacinumhas potential to produce some bioactive secondary metabolites againstB. cinerea. In this study, we applied matrix-assisted laser desorption ionization-time of flight mass spectrometry imaging mass spectrometry (MALDI-TOF-IMS), as a fast identification tool, for the discovery of a new antifungal lipopeptaibol (leucinostatin Z) fromP. lilacinumagainstB. cinerea. The planar structure of leucinostatin Z was further established by using the LC-HRESI-MS-MS analysis. MALDI-TOF-IMS is becoming a new approach that allows us to observe the bioactive natural products directly on growth media between the colonies of two fungi, which is faster and more effective than the traditional techniques to discover new bioactive compounds in fungi.