YocM a small heat shock protein can protect Bacillus subtilis cells during salt stress
YocM a small heat shock protein can protect Bacillus subtilis cells during salt stress
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DOI：
10.1111/mmi.14164
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发表时间：
2019-02-01
影响因子：
3.6
通讯作者：
Turgay, Kuersad
中科院分区：
文献类型：
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作者：
Hantke, Ingo;Schaefer, Heinrich;Turgay, Kuersad
小分子热休克蛋白（Small heat shock proteins，sHsp）广泛存在于生命的各个领域。通过与错误折叠或聚集的蛋白质相互作用，这些分子伴侣在细胞蛋白质稳态中发挥保护作用。在这里，我们证明了革兰氏阳性模式生物枯草芽孢杆菌的sHsp YocM是细胞蛋白质质量控制系统的一部分，在盐胁迫反应中具有特定的作用。在缺乏YocM的情况下，盐休克细胞的存活受损，并且YocM水平的增加保护B。枯草杆菌暴露于热或盐。我们观察到盐和热应激诱导的YocM定位到细胞内的蛋白质聚集体。有趣的是，纯化的YocM似乎在体外加速不同模型底物的蛋白质聚集。此外，YocM和化学分子伴侣的组合存在，其在盐应激细胞中积累，可以促进体外对蛋白质错误折叠的协同保护作用。因此，YocM在盐胁迫期间的有益作用可能与化学分子伴侣的相互功能关系有关，并为sHsp分子伴侣活性增加了一个新的可能的功能方面。
Small heat shock proteins (sHsp) occur in all domains of life. By interacting with misfolded or aggregated proteins these chaperones fulfill a protective role in cellular protein homeostasis. Here, we demonstrate that the sHsp YocM of the Gram-positive model organism Bacillus subtilis is part of the cellular protein quality control system with a specific role in salt stress response. In the absence of YocM the survival of salt shocked cells is impaired, and increased levels of YocM protect B. subtilis exposed to heat or salt. We observed a salt and heat stress-induced localization of YocM to intracellular protein aggregates. Interestingly, purified YocM appears to accelerate protein aggregation of different model substrates in vitro. In addition, the combined presence of YocM and chemical chaperones, which accumulate in salt stressed cells, can facilitate in vitro a synergistic protective effect on protein misfolding. Therefore, the beneficial role of YocM during salt stress could be related to a mutual functional relationship with chemical chaperones and adds a new possible functional aspect to sHsp chaperone activities.