Opioid-Mediated Astrocyte-Neuron Signaling in the Nucleus Accumbens
Opioid-Mediated Astrocyte-Neuron Signaling in the Nucleus Accumbens
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DOI：
10.3390/cells8060586
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发表时间：
2019-06-01
期刊：
影响因子：
6
通讯作者：
Araque, Alfonso
中科院分区：
文献类型：
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作者：
Corkrum, Michelle;Rothwell, Patrick E.;Araque, Alfonso
星形胶质细胞生理学的主要标志是响应神经递质的细胞内钙的升高和调节神经元活性的神经活性物质（胶质递质）的释放。虽然已经在脑桥核的星形胶质细胞中鉴定了μ-阿片样物质受体表达，但对星形胶质细胞-神经元通讯的功能后果在很大程度上仍然未知。本研究探讨了星形胶质细胞对μ-阿片信号的反应性和延髓核胶质传递的调节。通过在脑切片中钙成像和全细胞膜片钳电生理学的组合，我们已经发现星形胶质细胞中的μ-阿片受体活化升高星形胶质细胞胞质钙并刺激胶质递质谷氨酸的释放，其通过神经元N-甲基-D-天冬氨酸（NMDA）受体的活化引起缓慢的内向电流。这些结果表明，阿片样物质介导的星形胶质细胞-神经元信号转导的分子机制在脑桥核的存在。
Major hallmarks of astrocyte physiology are the elevation of intracellular calcium in response to neurotransmitters and the release of neuroactive substances (gliotransmitters) that modulate neuronal activity. While mu -opioid receptor expression has been identified in astrocytes of the nucleus accumbens, the functional consequences on astrocyte-neuron communication remains largely unknown. The present study has investigated the astrocyte responsiveness to mu -opioid signaling and the regulation of gliotransmission in the nucleus accumbens. Through the combination of calcium imaging and whole-cell patch clamp electrophysiology in brain slices, we have found that mu -opioid receptor activation in astrocytes elevates astrocyte cytoplasmic calcium and stimulates the release of the gliotransmitter glutamate, which evokes slow inward currents through the activation of neuronal N-methyl-D-aspartate (NMDA) receptors. These results indicate the existence of molecular mechanisms underlying opioid-mediated astrocyte-neuron signaling in the nucleus accumbens.