BDNF IN THE DEVELOPMENT OF THE VISUAL-SYSTEM OF XENOPUS
BDNF IN THE DEVELOPMENT OF THE VISUAL-SYSTEM OF XENOPUS
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DOI：
10.1016/0896-6273(94)90328-x
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发表时间：
1994-04-01
期刊：
影响因子：
16.2
通讯作者：
FRASER, SE
中科院分区：
文献类型：
--
作者：
COHENCORY, S;FRASER, SE
为探讨脑源性神经营养因子(BDNF)在非洲爪哇视觉系统发育中的作用，检测了BDNF和TrkB在体外培养的非洲爪哇视网膜神经节细胞(RGC)发育过程中的表达及BDNF对RGC发育的影响。BDNF mRNA在发育中的眼和顶盖均有表达，在45/46期达到高峰。BDNF的表达与TrkB全长转录本的RGC表达一致，提示BDNF具有功能作用。在培养条件下，BDNF可显著增加RGCs的数量。脑源性神经营养因子挽救投射到顶盖的分化的视网膜节细胞的能力、作用的时间进程和缺乏有丝分裂反应表明这种神经营养因子促进存活。BDNF和TrkB的表达以及RGCs对BDNF的反应性与视网膜轴突终末的分枝和图案化相一致。我们的结果表明，BDNF是一种与非洲爪哇RGC发育相关的神经营养因子，并提示它在视觉系统模式形成中发挥作用。
To explore the role of BDNF during Xenopus visual system development, the expression of BDNF and TrkB, as well as the effect of BDNF during retinal ganglion cell (RGC) development in culture, was examined. BDNF mRNA was found to be expressed in both the developing eye and tectum, peaking at stage 45/46. The expression of BDNF coincided with RGC expression of full-length trkB transcripts, suggesting a functional role for BDNF. In culture, BDNF significantly increased the number of RGCs. The ability of BDNF to rescue differentiated RGCs that had projected to the tectum, the time course of the effect, and the lack of mitogenic response indicate that this neurotrophin promotes survival. The expression of BDNF and TrkB and the responsiveness of RGCs to BDNF coincide with retinal axon terminal arborization and patterning. Our results indicate that BDNF is a relevant neurotrophin for Xenopus RGC development and suggest that it plays a role during visual system patterning.