SPECIFIC TRANSLATIONAL ACTIVATION BY NUCLEAR GENE-PRODUCTS OCCURS IN THE 5' UNTRANSLATED LEADER OF A YEAST MITOCHONDRIAL MESSENGER-RNA
SPECIFIC TRANSLATIONAL ACTIVATION BY NUCLEAR GENE-PRODUCTS OCCURS IN THE 5' UNTRANSLATED LEADER OF A YEAST MITOCHONDRIAL MESSENGER-RNA
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DOI：
10.1073/pnas.85.8.2677
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发表时间：
1988-04-01
影响因子：
11.1
通讯作者：
FOX, TD
中科院分区：
文献类型：
--
作者：
COSTANZO, MC;FOX, TD
酵母线粒体编码细胞色素C氧化酶亚基III(CoxIII)的mRNA的翻译被至少三个核基因PET494、PET54和PET122的产物特异性激活。为了研究翻译激活的目标位置是否在coxIII mRNA的5‘’未翻译前导内，我们询问了从含有coxIII mRNA前导的嵌合mRNA中翻译另一种线粒体蛋白apo-细胞色素b是否需要PET494、PET54或PET122。这三个基因中的任何一个突变都会导致细胞色素b从带有5‘’三分之二的coxIII mRNA5‘’未翻译前导的mRNA中消失，这表明所有这三个基因产物都是翻译嵌合mRNA所必需的，并且必须在coxIII mRNA5‘’2‘前导的5’‘三分之二范围内起作用。我们的数据表明，在野生型细胞中，PET494、PET54和PET122对CoxIII翻译的特异性激活是通过这三种基因产物在起始密码子上游至少172个核苷酸的5‘’未翻译前导区域的一个或多个位点上的作用而发生的。
Translation of the yeast mitochondrial mRNA encoding cytochrome c oxidase subunit III (coxIII) is specifically activated by the products of at least three nuclear genes, PET494, PET54, and PET122. To investigate whether the target site for translational activation is within the 5'' untranslated leader of the coxIII mRNA, we asked whether translation of another mitochondrial protein, apo-cytochrome b, from a chimeric mRNA bearing the coxIII mRNA leader required PET494, PET54, or PET122. Mutations in any of these three genes abolished translation of cytochrome b from an mRNA bearing the 5'' two-thirds of the coxIII mRNA 5'' untranslated leader, showing that all three gene products are required for translation of the chimeric mRNA and must act within the 5'' two-thirds of the coxIII mRNA leader. Our data suggest that in wild-type cells, the specific activation of coxIII translation by PET494, PET54, and PET122 occurs by the action of these three gene products at a site or sites in a region of the 5'' untranslated leader at least 172 nucleotides upstream of the initiation codon.