Integration of a mutant c-Ha-ras oncogene into C3H/10T1/2 cells and its relationship to tumorigenic transformation.
Integration of a mutant c-Ha-ras oncogene into C3H/10T1/2 cells and its relationship to tumorigenic transformation.
复制标题
突变 c-Ha-ras 癌基因整合到 C3H/10T1/2 细胞中及其与致瘤转化的关系。
DOI：
10.1093/carcin/6.9.1295
复制
发表时间：
1985
期刊：
影响因子：
4.7
通讯作者：
Fahl,WE
中科院分区：
文献类型：
--
作者：
Manoharan,TH;Burgess,JA;Ho,D;Newell,CL;Fahl,WE
C3H/10T1/2-CL8小鼠细胞在DNA转染频率为（2 - 6 × 10 - 4）的情况下接受并表达质粒克隆的耐药基因（Ecogpt），这对于基因转化群体的常规恢复是可以接受的。用突变的c- ha -理性癌基因（pEJ6.6质粒）转染10T1/2细胞，通过裸鼠的集落形态和致瘤性生长来判断，10T1/2细胞群发生了肿瘤转化。测定了c-Ha-rasgene突变体在致瘤细胞群和10T1/2细胞对照中的整合和表达水平，在大多数致瘤细胞群中发现了最高水平的突变转录。对10T1/2和NIH/3T3细胞的初步比较显示，pej6.6诱导的转化灶频率相似，对克隆的b -淋巴原基因的转化效果缺乏敏感性。结果确定了一个遗传事件，先前已被证明是致癌物质诱导的，它允许广泛使用的致癌物质转化的10T1/2小鼠细胞系的肿瘤转化。
C3H/10T1/2-CL8 mouse cells were shown to take up and express a plasmid-cloned drug resistance gene (Ecogpt) after DNA transfection at a frequency (2−6 × 10−4) which is acceptable for routine recovery of gene-transformed populations. Transfection of 10T1/2 cells with a mutant c-Ha-rasoncogene (pEJ6.6 plasmid) results in neoplastically transformed 10T1/2 cell populations as judged by colony morphology and tumorigenic growth in nude mice. The levels of mutant c-Ha-rasgene integration and expression in the tumorigenic cell populations and 10T1/2 cell controls were determined, and the highest level of mutantrastranscript was seen in the most tumorigenic cell population. A preliminary comparison of 10T1/2 and NIH/3T3 cells showed similar frequencies for pEJ6.6-induced transformed foci and a similar lack of sensitivity to the transforming effects of a cloned B-lymoncogene. The results identify a genetic event, which has previously been shown to be carcinogen-inducible, that is permissive for neoplastic transformation of the widely used carcinogen-transformable 10T1/2 mouse cell line.