Effects of hepatocyte growth factor on viability and biotransformation functions of hepatocytes in gel entrapped and monolayer culture.
Effects of hepatocyte growth factor on viability and biotransformation functions of hepatocytes in gel entrapped and monolayer culture.
复制标题
肝细胞生长因子对凝胶包埋和单层培养中肝细胞活力和生物转化功能的影响。
DOI：
10.1097/00003246-199507000-00014
复制
发表时间：
1995
影响因子：
8.8
通讯作者：
Cerra,FB
中科院分区：
文献类型：
--
作者：
Hu,MY;Cipolle,M;Sielaff,T;Lovdahl,MJ;Mann,HJ;Remmel,RP;Cerra,FB
目的体外生物人工肝装置必须能维持高密度培养的肝细胞的活力和分化功能。生长因子，如肝细胞生长因子，在暴发性肝衰竭患者的血浆中发现高浓度，有可能促进肝细胞去分化，从而降低功能。我们验证了肝细胞生长因子可以提高单层培养和体外生物人工肝中胶原柱状凝胶“面条”包裹的肝细胞的活细胞密度，降低肝细胞的生物转化功能。设计
ObjectivesAn extracorporeal bioartificial liver device must maintain viability and differentiated function of hepatocytes cultivated at high cell density. Growth factors, such as hepatocyte growth factor, found in high concentrations in the plasma of patients with fulminant hepatic failure, have the potential to promote hepatocyte dedifferentiation and thus, decrease function. We tested the hypothesis that hepatocyte growth factor would improve viable cell density and decrease biotrans-formation functions of liver cells in monolayer culture and in hepatocytes entrapped in collagen cylindrical gel``noodles''as found in the extracorporeal bioartificial liver.Design