Surfactant protein D interacts with Pneumocystis carinii and mediates organism adherence to alveolar macrophages.
Surfactant protein D interacts with Pneumocystis carinii and mediates organism adherence to alveolar macrophages.
复制标题
表面活性蛋白 D 与卡氏肺囊虫相互作用并介导生物体粘附到肺泡巨噬细胞。
DOI：
10.1172/jci117972
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Limper,AH
中科院分区：
文献类型：
--
作者：
O'Riordan,DM;Standing,JE;Kwon,KY;Chang,D;Crouch,EC;Limper,AH
卡氏肺孢子虫通过其富含甘露糖的表面抗原复合物（称为gpA）与下呼吸道中的糖蛋白相互作用。表面活性剂蛋白D（SP-D）是最近描述的空域衬里材料的组分，其具有能够与存在于微生物和白细胞上的糖缀合物相互作用的钙依赖性凝集素结构域。因此，我们在免疫抑制大鼠模型中评估肺孢子虫肺炎期间SP-D在下呼吸道中的程度和定位，并检查其在调节卡氏肺孢子虫与巨噬细胞相互作用中的作用。我们报告说，SP-D是肺泡渗出物的一个主要组成部分，代表卡氏肺孢子虫肺炎，是目前结合到卡氏肺孢子虫生物体的表面在体内。我们进一步证明SP-D通过糖介导的与存在于生物体表面上的gpA的相互作用与卡氏肺孢子虫结合。最后，我们表明SP-D增强卡氏肺孢子虫与肺泡巨噬细胞的结合，但不显著增强巨噬细胞对生物体的吞噬作用。SP-D与gpA的相互作用代表了卡氏肺孢子虫肺炎期间宿主-寄生虫关系的另一个重要组成部分。图片
Pneumocystis carinii interacts with glycoproteins present in the lower respiratory tract through its mannose-rich surface antigen complex termed gpA. Surfactant protein D (SP-D) is a recently described component of the airspace lining material that possesses a calcium-dependent lectin domain capable of interacting with glycoconjugates present on microorganisms and leukocytes. Accordingly, we evaluated the extent and localization of SP-D in the lower respiratory tract during Pneumocystis pneumonia in an immunosuppressed rat model and examined its role in modulating interaction of P. carinii with macrophages. We report that SP-D is a major component of the alveolar exudates that typify P. carinii pneumonia and is present bound to the surface of P. carinii organisms in vivo. We further demonstrate that SP-D binds to P. carinii through saccharide-mediated interactions with gpA present on the surface of the organism. Lastly, we show that SP-D augments binding of P. carinii to alveolar macrophages, but does not significantly enhance macrophage phagocytosis of the organism. The interaction of SP-D with gpA represents an additional important component of the host-parasite relationship during P. carinii pneumonia.Images