FtMYB6, a Light-Induced SG7 R2R3-MYB Transcription Factor, Promotes Flavonol Biosynthesis in Tartary Buckwheat (Fagopyrum tataricum)
FtMYB6, a Light-Induced SG7 R2R3-MYB Transcription Factor, Promotes Flavonol Biosynthesis in Tartary Buckwheat (Fagopyrum tataricum)
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FtMYB6 是一种光诱导 SG7 R2R3-MYB 转录因子，可促进苦荞 (Fagopyrum tataricum) 中的黄酮醇生物合成
DOI：
10.1021/acs.jafc.0c03037
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发表时间：
2020
影响因子：
6.1
通讯作者：
Haixia Zhao
中科院分区：
文献类型：
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作者：
Pangfeng Yao;Yunji Huang;Qixin Dong;Min Wan;Anhu Wang;Yuwei Chen;Chenglei Li;Qi Wu;Hui Chen;Haixia Zhao
苦荞（Fagopyrum tararicum）富含黄酮醇，被认为对人体健康非常有益。然而，对苦荞黄酮醇生物合成的调控机制知之甚少。在这项研究中，我们鉴定并鉴定了苦荞中一个新的SG7 R2R3-MYB转录因子FtMYB6。我们发现ftmyb6位于细胞核中，并作为转录激活因子。ftmyb6启动子具有较强的时空特异性，可由光诱导。offtmyb6的表达与芦丁在根、茎、叶和花中的积累有显著的相关性。在转基因苦荞毛状根和烟草植株中过表达offtmyb6显著增加了黄酮醇的积累。在瞬时荧光素酶（LUC）活性测定中，FtMYB6促进了offtf3handftfls1启动子的活性，抑制了ft4cl启动子的活性。综上所述，我们的研究结果表明FtMYB6通过激活ftf3handftfls1表达来促进黄酮醇的生物合成。
Tartary buckwheat (Fagopyrum tataricum) is rich in flavonols, which are thought to be highly beneficial for human health. However, little is known about the regulatory mechanism of flavonol biosynthesis in Tartary buckwheat. In this study, we identified and characterized a novel SG7 R2R3-MYB transcription factor in Tartary buckwheat, FtMYB6. We showed thatFtMYB6is located in the nucleus and acts as a transcriptional activator. TheFtMYB6promoter showed strong spatiotemporal specificity and was induced by light. The expression ofFtMYB6showed a significant correlation with rutin accumulation in the roots, stems, leaves, and flowers. Overexpression ofFtMYB6in transgenic Tartary buckwheat hairy roots and tobacco (Nicotiana tabacum) plants significantly increased the accumulation of flavonols. In transient luciferase (LUC) activity assay, FtMYB6 promoted the activity ofFtF3HandFtFLS1promoters and inhibited the activity of theFt4CLpromoter. Collectively, our results suggest that FtMYB6 promotes flavonol biosynthesis by activatingFtF3HandFtFLS1expression.