Effects of isoflurane on learning and memory functions of wild-type and glutamate transporter type 3 knockout mice
Effects of isoflurane on learning and memory functions of wild-type and glutamate transporter type 3 knockout mice
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DOI：
10.1111/j.2042-7158.2011.01404.x
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发表时间：
2012-02-01
影响因子：
3.3
通讯作者：
Zuo, Zhiyi
中科院分区：
文献类型：
--
作者：
Lee, Sunam;Park, Sang-Hon;Zuo, Zhiyi
目的 全身麻醉可能会导致术后认知功能障碍。本研究旨在确定异氟醚对健康动物或大脑抗氧化能力降低的动物的学习和记忆的影响。方法将大脑谷胱甘肽水平降低的7至9周龄雌性CD-1野生型小鼠或谷氨酸转运蛋白3型（EAAT3）敲除小鼠暴露于或不暴露于1.3%异氟醚2小时。 1周后，他们接受了恐惧调节或巴恩斯迷宫测试。 主要发现 EAAT3基因敲除小鼠在与音调相关的恐惧中比野生型小鼠有更少的冻结行为。异氟烷不影响野生型和 EAAT3 敲除小鼠的冷冻行为。野生型和EAAT3敲除小鼠在巴恩斯迷宫训练和记忆测试中识别目标孔的时间不受异氟烷影响。然而，在这些测试中，EAAT3敲除小鼠比野生型小鼠需要更长的时间来识别目标孔。结论这些结果表明EAAT3敲除小鼠具有显着的认知障碍。异氟烷可能不会显着影响野生型和 EAAT3 敲除小鼠在异氟烷暴露后延迟阶段的认知能力。
Objectives General anesthetics may contribute to the post-operative cognitive dysfunction. This study was designed to determine the effects of isoflurane on the learning and memory of healthy animals or animals with a decreased brain antioxidative capacity.Methods Seven-to nine-week-old female CD-1 wild-type mice or glutamate transporter type 3 (EAAT3) knockout mice whose brains have a decreased glutathione level were exposed to or were not exposed to 1.3% isoflurane for 2 h. They were subjected to fear conditioning or Barnes maze tests 1 week later.Key findings The EAAT3 knockout mice had less freezing behaviour than the wild-type mice in tone-related fear. Isoflurane did not affect the freezing behaviour of the wild-type and EAAT3 knockout mice. The time for the wild-type and EAAT3 knockout mice to identify the target hole in the training sessions and memory test with the Barnes maze was not affected by isoflurane. However, the EAAT3 knockout mice took longer to identify the target hole than the wild-type mice in these tests.Conclusions These results suggest that EAAT3 knockout mice have significant cognitive impairment. Isoflurane may not significantly affect the cognition of wild-type and EAAT3 knockout mice in a delayed phase after isoflurane exposure.