LONG-TERM CULTURE AND FUNCTIONAL-CHARACTERIZATION OF FOLLICULAR CELLS FROM ADULT NORMAL HUMAN THYROIDS
LONG-TERM CULTURE AND FUNCTIONAL-CHARACTERIZATION OF FOLLICULAR CELLS FROM ADULT NORMAL HUMAN THYROIDS
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DOI：
10.1073/pnas.91.19.9004
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发表时间：
1994-09-13
影响因子：
11.1
通讯作者：
COON, HG
中科院分区：
文献类型：
--
作者：
CURCIO, F;AMBESIIMPIOMBATO, FS;COON, HG
我们已经从正常成人甲状腺中获得了分化的增殖滤泡细胞的长期培养物。这种人类细胞的体外生长是通过改良的 F-12 培养基维持的，该培养基补充了牛下丘脑和垂体提取物，并且不添加促甲状腺素。培养物已被扩增、克隆、冷冻、成功回收和表征。这些细胞的功能特征显示组成型甲状腺球蛋白的产生和释放以及促甲状腺素依赖性腺苷3',5'-环单磷酸的产生，后者显然与DNA合成或细胞增殖的显着增加无关。通过染色体计数对这些细胞进行遗传表征，显示出正常的二倍体染色体数目。在长期培养中培养分化的人甲状腺滤泡细胞的能力为研究正常和病理人体组织中促甲状腺素刺激的转导途径、开发临床相关的体外测定以及考虑细胞和分子疗法提供了可能性。
We have obtained long-term cultures of differentiated proliferating follicular cells from normal adult human thyroid glands. In vitro growth of such human cells has been sustained by a modified F-12 medium, supplemented with bovine hypothalamus and pituitary extracts and no added thyrotropin. Cultures have been expanded, cloned, frozen, successfully retrieved, and characterized. Functional characterization of these cells shows constitutive thyroglobulin production and release and thyrotropin-dependent adenosine 3',5'-cyclic monophosphate production, the latter apparently not associated with significant increases in DNA synthesis or cell proliferation. Genetic characterization of these cells by chromosome counting showed the normal diploid chromosome number. The ability to cultivate differentiated human thyroid follicular cells in long-term culture opens possibilities for investigating the transduction pathways of thyrotropin stimulation in normal and pathological human tissues, developing clinically relevant in vitro assays, and considering cellular and molecular therapies.