Maturation of nematode-induced galls in Medicago truncatula is related to water status and primary metabolism modifications
Maturation of nematode-induced galls in Medicago truncatula is related to water status and primary metabolism modifications
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DOI：
10.1016/j.plantsci.2014.12.019
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发表时间：
2015-03-01
期刊：
影响因子：
5.2
通讯作者：
Frendo, Pierre
中科院分区：
文献类型：
--
作者：
Baldacci-Cresp, Fabien;Maucourt, Mickael;Frendo, Pierre
根结线虫是专性植物寄生蠕虫，与寄主植物建立并维持密切关系。在一个兼容的相互作用，这些线虫诱导根细胞再分化成多核和肥大的巨细胞（GC）。这些代谢活跃的摄食细胞构成了线虫唯一的营养来源。分析了南方根结线虫（Meloidogyne incognita）诱导的蒺藜状苜蓿（Medicago truncatula）幼根发育和成熟虫瘿中水分状况、离子含量和代谢产物积累的变化。水势和渗透压显着修改在成熟的虫瘿相比，发展中的虫瘿和控制根。离子含量显着修改虫瘿相比，根。主成分分析的代谢物含量表明，成熟的胆汁代谢显着修改相比，发展胆汁代谢。最显著的差异是海藻糖含量增加了三倍，而成熟的虫瘿中葡萄糖浓度减少了五倍。基因表达分析表明，海藻糖的积累，至少，部分地与一个显着较低的表达海藻糖酶基因在成熟的虫瘿。我们的研究结果指出，显着的修改胆囊生理成熟过程中。(C)由Elsevier爱尔兰有限公司出版。
Root-knot nematodes are obligatory plant parasitic worms that establish and maintain an intimate relationship with their host plants. During a compatible interaction, these nematodes induce the redifferentiation of root cells into multinucleate and hypertrophied giant cells (GCs). These metabolically active feeding cells constitute the exclusive source of nutrients for the nematode. We analyzed the modifications of water status, ionic content and accumulation of metabolites in development and mature galls induced by Meloidogyne incognita and in uninfected roots of Medicago truncatula plants. Water potential and osmotic pressure are significantly modified in mature galls compared to developing galls and control roots. Ionic content is significantly modified in galls compared to roots. Principal component analyses of metabolite content showed that mature gall metabolism is significantly modified compared to developing gall metabolism. The most striking differences were the three-fold increase of trehalose content associated to the five-fold diminution in glucose concentration in mature galls. Gene expression analysis showed that trehalose accumulation was, at least, partially linked to a significantly lower expression of the trehalase gene in mature galls. Our results point to significant modifications of gall physiology during maturation. (C) 2015 Published by Elsevier Ireland Ltd.