Integrated cytogenetic map of mitotic metaphase chromosome 9 of maize: resolution, sensitivity, and banding paint development
Integrated cytogenetic map of mitotic metaphase chromosome 9 of maize: resolution, sensitivity, and banding paint development
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DOI：
10.1007/s00412-008-0151-y
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发表时间：
2008-08-01
期刊：
影响因子：
1.6
通讯作者：
Birchler, James A.
中科院分区：
文献类型：
--
作者：
Danilova, Tatiana V.;Birchler, James A.
为了研究物理 DNA 指纹重叠群 (FPC) 图和粗线期和体细胞玉米染色体的细胞遗传学图上序列位置的相关性，使用荧光原位杂交 (FISH) 选择沿 9 号染色体 FPC 图大约每 10 Mb 的序列放置在玉米染色体上。探针是根据遗传标记序列或绘制的细菌人工染色体（BAC）克隆的无重复部分的聚合酶链式反应产物而产生的。在 9 号染色体上观察到 15 个探针。除了一些位于着丝粒周围区域的探针外，大多数序列的细胞学位置与粗线期、体细胞和 FPC 图相对应。由于有丝分裂中期染色体的不均匀凝结，着丝粒周围区域较低，臂中较高，探针位置移位到双臂的远端。体细胞 9 号染色体上 FISH 的轴向分辨率为 3.3 至 8.2 Mb，比粗线期染色体低 12-30 倍。探针集合可用作染色体标志或“条带涂料”，用于对包括转基因和 BAC 克隆在内的序列进行物理作图，并用于研究染色体重排。
To study the correlation of the sequence positions on the physical DNA finger print contig (FPC) map and cytogenetic maps of pachytene and somatic maize chromosomes, sequences located along the chromosome 9 FPC map approximately every 10 Mb were selected to place on maize chromosomes using fluorescent in situ hybridization (FISH). The probes were produced as pooled polymerase chain reaction products based on sequences of genetic markers or repeat-free portions of mapped bacterial artificial chromosome (BAC) clones. Fifteen probes were visualized on chromosome 9. The cytological positions of most sequences correspond on the pachytene, somatic, and FPC maps except some probes at the pericentromeric regions. Because of unequal condensation of mitotic metaphase chromosomes, being lower at pericentromeric regions and higher in the arms, probe positions are displaced to the distal ends of both arms. The axial resolution of FISH on somatic chromosome 9 varied from 3.3 to 8.2 Mb, which is 12-30 times lower than on pachytene chromosomes. The probe collection can be used as chromosomal landmarks or as a "banding paint" for the physical mapping of sequences including transgenes and BAC clones and for studying chromosomal rearrangements.