Labelling and optical erasure of synaptic memory traces in the motor cortex.
Labelling and optical erasure of synaptic memory traces in the motor cortex.
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DOI：
10.1038/nature15257
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发表时间：
2015-09-17
期刊：
影响因子：
64.8
通讯作者：
Kasai H
中科院分区：
文献类型：
--
作者：
Hayashi-Takagi A;Yagishita S;Nakamura M;Shirai F;Wu YI;Loshbaugh AL;Kuhlman B;Hahn KM;Kasai H
树突棘是突触可塑性的主要位点，并被认为可能是记忆的结构相关物。然而，对大脑皮层中特定树突棘子集进行系统性操作一直无法实现，因此，树突棘与记忆之间的联系只是相关性的。我们开发了一种新型突触光探针，即AS - PaRac1（靶向可光激活Rac1的激活突触），它能够特异性标记近期增强的树突棘，并诱导含有AS - PaRac1的树突棘选择性收缩。对AS - PaRac1的体内成像显示，运动学习会在一小部分神经元中引发大量的突触重塑。通过光学手段使增强的树突棘收缩会破坏已获得的运动学习能力，而在同一皮层区域中由不同运动任务诱发的树突棘进行相同操作却不会影响运动学习能力。综上所述，我们的研究结果表明，新获得的运动技能依赖于特定任务的密集突触集合体的形成。
Dendritic spines are the major loci of synaptic plasticity and are considered as possible structural correlates of memory. Nonetheless, systematic manipulation of specific subsets of spines in the cortex has been unattainable, and thus, the link between spines and memory has been correlational. We developed a novel synaptic optoprobe, AS-PaRac1 (activated synapse targeting photoactivatable Rac1), which can label recently potentiated spines specifically, and induce the selective shrinkage of AS-PaRac1-containing spines. In vivo imaging of AS-PaRac1 revealed that a motor learning induced substantial synaptic remodelling in a small subset of neurons. The acquired motor learning was disrupted by the optical shrinkage of the potentiated spines, whereas it was not affected by the identical manipulation of spines evoked by a distinct motor task in the same cortical region. Taken together, our results demonstrate that a newly acquired motor skill depends on the formation of a task-specific dense synaptic ensemble.