Delayed cuticular penetration and enhanced metabolism of deltamethrin in pyrethroid-resistant strains of Helicoverpa armigera from China and Pakistan
Delayed cuticular penetration and enhanced metabolism of deltamethrin in pyrethroid-resistant strains of Helicoverpa armigera from China and Pakistan
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DOI：
10.1002/ps.1225
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发表时间：
2006-09-01
影响因子：
4.1
通讯作者：
Bromilow, Richard H.
中科院分区：
文献类型：
--
作者：
Ahmad, Mushtaq;Denholm, Ian;Bromilow, Richard H.
本文研究了[C-14]溴氰菊酯在中国棉铃虫（Helicoverpaarmigera）和巴基斯坦棉铃虫（Helicoverpaarmigera（Hubner））敏感品系和抗性品系中的渗透和代谢。在24小时内，溴氰菊酯渗透到抗性个体中的速度明显慢于敏感个体。对于敏感品系和两种抗性品系，施用溴氰菊酯的50%渗透所需时间分别为1 h和6 h。抗性品系的幼虫比敏感品系的幼虫更快地降低内放射性。48 h后，40%的渗透溴氰菊酯在敏感品系的幼虫体内变硬，而巴基斯坦品系为1.5-5%，中国品系为8-14%。两种耐药菌株都产生甲醇可溶性和水溶性代谢物，但敏感菌株仅产生甲醇可溶性代谢物。给药后12、24和48 h，耐药菌株分泌的甲醇可溶性代谢物的量几乎是敏感菌株的两倍。两种耐药菌株在48 h后也以水溶性代谢物的形式排出5-7%的渗透剂量。与中国品系相比，巴基斯坦品系对溴氰菊酯的穿透速度慢，内含量低，排泄速度快，这与巴基斯坦品系的抗性较高有关。这些发现表明，巴基斯坦和中国的耐药H。棉铃虫具有减少表皮渗透和增强溴氰菊酯和其他拟除虫菊酯代谢的机制。(c)2006化学工业协会。
The penetration and metabolism of [C-14]deltamethrin was studied in susceptible and resistant Chinese and Pakistani strains of Helicoverpa armigera (Hubner), which were resistant to deltamethrin by 330- and 670-fold, respectively. The penetration of deltamethrin into resistant individuals was significantly slower than into susceptible individuals over a 24-h period. The time taken for 50% penetration of the applied deltamethrin was I h for the susceptible strain and 6 h for both of the resistant strains. The internal radioactivity was reduced by the larvae of resistant strains much faster than by the susceptible larvae. After 48 h, 40% of the penetrated deltamethrin was stiff inside the larvae of the susceptible strain, in comparison with 1.5-5% in the Pakistani strain and 8-14% in the Chinese strain. Both of the resistant strains produced methanol-soluble and water-soluble metabolites, but the susceptible strain produced methanol-soluble metabolites only. By 12, 24 and 48 h after dosing, the amount of methanol-soluble metabolites excreted by the resistant strains was almost double that of the susceptible strain. Both of the resistant strains also excreted 5-7% of the penetrated dose as a water-soluble metabolite after 48 h. In comparison with the Chinese strain, the Pakistani strain exhibited slower penetration, lower internal content and faster excretion of deltamethrin, which correlated with the higher resistance of the Pakistani strain. These findings show that the resistant Pakistani and Chinese strains of H. armigera possess mechanisms of reduced cuticular penetration and enhanced metabolism of deltamethrin and perhaps other pyrethroids. (c) 2006 Society of Chemical Industry.