Schistosoma mansoni: susceptibility differences between male and female mice can be mediated by testosterone during early infection.
Schistosoma mansoni: susceptibility differences between male and female mice can be mediated by testosterone during early infection.
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DOI：
10.1006/expr.1997.4148
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发表时间：
1997-03
影响因子：
2.1
通讯作者：
M. Nakazawa;M. Fantappíé;G. Freeman;S. Elói-Santos;N. Olsen;W. Kovacs;W. Secor;D. Colley
中科院分区：
文献类型：
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作者：
M. Nakazawa;M. Fantappíé;G. Freeman;S. Elói-Santos;N. Olsen;W. Kovacs;W. Secor;D. Colley
在小鼠曼氏血吸虫感染中，感染相同数量尾蚴的雄性蠕虫比雌性小鼠发育较少的成虫。为了评估睾酮在这种现象中的潜在作用，在CBA/J小鼠组中操纵睾酮水平，然后感染并监测存活率，蠕虫负担，器官肿大和卵子产生。感染16周后，睾酮水平较低的组（未处理的雌性、去势雄性或载体处理的去势小鼠）中超过80%的小鼠死亡，而睾酮水平较高的组（假去势雄性、睾酮处理的去势小鼠或睾酮处理的雌性小鼠）中不到40%的小鼠死于感染。从低睾酮水平组小鼠中回收的蠕虫平均数量在组间相当，并且显著大于高睾酮水平组。观察到的器官肿大程度与蠕虫负荷密切相关，但各组间每只雌性蠕虫的肝卵数量无显著差异。雄性小鼠在S.曼氏感染后，宿主存活率无显著差异。此外，如果在感染前10天给予睾酮，但如果在感染后10天或5周给予睾酮，则用睾酮治疗的雌性小鼠表现出蠕虫负担减少。因此，在平行感染的雄性和雌性小鼠中观察到的宿主性别偏倚似乎与感染早期，在未成熟的精子体发育期间存在雄性性腺组织或睾酮有关。
In murine Schistosoma mansoni infections, fewer adult worms develop in male than in female mice infected with the same number of cercariae. To evaluate a potential role for testosterone in this phenomenon, testosterone levels were manipulated in groups of CBA/J mice that were then infected and monitored for survival rates, worm burdens, organomegaly, and egg production. By 16 weeks of infection, more than 80% of mice in groups with low levels of testosterone (untreated females, castrated males, or carrier-treated castrates) were dead, while less than 40% of those in groups with high levels of testosterone (sham-castrated males, testosterone-treated castrates, or testosterone-treated female mice) succumbed to infection. The mean number of worms recovered from mice in the low testosterone level groups was comparable among groups, and significantly greater than that from those in high-testosterone-level groups. The degree of organomegaly observed correlated strongly with worm burden, but the number of hepatic eggs per female worm did not differ significantly between groups. When male mice were castrated or sham-castrated 5 weeks after S. mansoni infection, no significant differences in host survival occurred. Furthermore, female mice treated with testosterone demonstrated reduced worm burdens if the testosterone was given 10 days prior to infection but not if the testosterone was given 10 days or 5 weeks after infection. Thus, the host sex bias observed in parallel-infected male and female mice appears to be related to the presence of male gonadal tissue or testosterone early in infection, during the development of immature schistosomules.