An odorant receptor and glomerulus responding to farnesene in Helicoverpa assulta (Lepidoptera: Noctuidae)
An odorant receptor and glomerulus responding to farnesene in Helicoverpa assulta (Lepidoptera: Noctuidae)
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Helicoverpa assulta（鳞翅目：夜蛾科）中的气味受体和肾小球对法呢烯有反应
DOI：
10.1016/j.ibmb.2018.11.006
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发表时间：
2018
影响因子：
3.8
通讯作者：
Wang Chen-Zhu
中科院分区：
文献类型：
--
作者：
Wu Han;Li Rui-Ting;Dong Jun-Feng;Jiang Nan-Ji;Huang Ling-Qiao;Wang Chen-Zhu
研究发现，虫害植物释放的萜类化合物在调节三营养相互作用中起重要作用。食草动物及其天敌如何感知萜类化合物至今尚未完全阐明。利用活体钙显像技术，我们发现法尼烯激活了烟青虫触角叶中的一个肾小球。由法尼烯异构体的混合物诱导的反应比由单独的E-β-法尼烯引起的反应更强。在非洲爪蟾卵母细胞表达系统中，HassOR 23/ORco被狭隘地调整为法尼烯异构体和具有相似结构的化合物。最后，行为学研究表明，法尼烯异构体对雌蛾的产卵有抑制作用。而对该虫的主要内寄生蜂-绿柄蜂Campoletischlorideae的寄主搜索有引诱作用。侮辱这些结果表明，法呢烯异构体编码的标记线模式在女性的嗅觉系统。assulta，表明法尼烯作为一种化学信号，从植物有重要的行为相关性和进化的影响，在三营养环境。
Terpenoids emitted from herbivore-damaged plants were found to play an important role in regulating tritrophic interactions. How herbivores and their natural enemies perceive terpenoids has not been thoroughly elucidated to date. Usingin vivocalcium imaging, we found in this study that farnesene activates one glomerulus in the antennal lobe of female Helicoverpa assulta. The response induced by a mixture of farnesene isomers is stronger than that elicited by E-β-farnesene alone. In the Xenopus oocyte expression system, HassOR23/ORco is narrowly tuned to farnesene isomers and compounds with similar structures. Finally, the behavioral studies showed that the farnesene isomers have an inhibitory effect on oviposition of femaleH. assulta, but have an attractive effect on host searching ofCampoletis chlorideae,the key endoparasitoid ofH. assultalarvae. These results demonstrate that farnesene isomers are encoded by a labeled-line mode in the olfactory system of femaleH. assulta, suggesting that farnesene as a chemical signal from plants has important behavioral relevance and evolutionary implications in the tritrophic context.