Immunohistochemical characterization of prototypical endometrial clear cell carcinoma-diagnostic utility of HNF-1β and oestrogen receptor
Immunohistochemical characterization of prototypical endometrial clear cell carcinoma-diagnostic utility of HNF-1β and oestrogen receptor
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DOI：
10.1111/his.12286
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发表时间：
2014-03-01
期刊：
影响因子：
6.4
通讯作者：
Lee, Cheng-Han
中科院分区：
文献类型：
--
作者：
Hoang, Lien N.;Han, Guangming;Lee, Cheng-Han
目的：绝大多数卵巢透明细胞癌的免疫组织化学特征为肝细胞核因子1同源盒B（HNF-1）阳性，雌激素受体（ER）阴性。然而，子宫内膜透明细胞癌中HNF-1和ER免疫染色的模式以及该面板在区分透明细胞癌与其他组织学类型的子宫内膜癌中的有用性尚未得到很好的defined.Methods和results我们检测了15例形态学经典的纯子宫内膜透明细胞癌中HNF-1、ER和p53的免疫染色模式，并与15例子宫内膜类浆液性癌和15例浆液性癌进行比较。我们观察到15例透明细胞癌中有14例出现弥漫性（>70%）中强核HNF-1染色和阴性ER染色，其余病例显示弥漫性强核HNF-1染色和局灶性ER染色。相比之下，15例浆液性癌中只有1例和15例类浆液性癌中无1例表现为弥漫性中度至强HNF-1核染色和阴性ER染色的组合。异常的p53免疫染色观察到5 15（33%）透明细胞carcinomas.ConclusionsOverall，我们的研究结果表明，类似的情况卵巢，HNF-1和ER的诊断面板可以被认为是透明细胞癌从子宫内膜浆液性癌和浆液性癌分离。
AimsThe great majority of ovarian clear cell carcinomas have a hepatocyte nuclear factor 1 homeobox B (HNF-1)-positive and oestrogen receptor (ER)-negative immunoprofile. However, the pattern of HNF-1 and ER immunostaining in clear cell carcinomas of the endometrium and the usefulness of this panel in distinguishing clear cell carcinoma from other histological types of endometrial carcinoma have yet to be well defined.Methods and resultsWe examined the immunostaining patterns of HNF-1, ER and p53 in 15 morphologically classic pure endometrial clear cell carcinomas, and compared these patterns with 15 endometrioid and 15 serous carcinomas of the endometrium. We observed the presence of diffuse (>70%) moderate to strong nuclear HNF-1 staining and negative ER staining in 14 of 15 clear cell carcinomas, with the remaining case showing both diffuse strong nuclear HNF-1 staining and focal ER staining. In comparison, only one of 15 serous carcinomas and none of 15 endometrioid carcinomas showed a combination of diffuse moderate to strong HNF-1 nuclear staining and negative ER staining. Aberrant p53 immunostaining was observed in five of 15 (33%) clear cell carcinomas.ConclusionsOverall, our findings demonstrate that, similarly to the situation for the ovary, a diagnostic panel of HNF-1 and ER may be considered for separating clear cell carcinoma from endometrioid and serous carcinoma of the endometrium.