Serine/threonine kinase gene Stpk-V, a key member of powdery mildew resistance gene Pm21, confers powdery mildew resistance in wheat
Serine/threonine kinase gene Stpk-V, a key member of powdery mildew resistance gene Pm21, confers powdery mildew resistance in wheat
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DOI：
10.1073/pnas.1016981108
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发表时间：
2011-05-10
影响因子：
11.1
通讯作者：
Chen, Peidu
中科院分区：
文献类型：
--
作者：
Cao, Aizhong;Xing, Liping;Chen, Peidu
Pm21基因位于簇毛麦染色体6V短臂上，以6VS.6AL易位（T6VS.6AL）的形式转入小麦，对小麦白粉病具有持久的广谱抗性。Pm21已成为世界范围内抗白粉病育种的重要基因资源。在中国，自2002年以来，12个含有Pm21的小麦品种已经种植了340多万公顷。由于6VS不能与6AS配对和重组，因此Pm21难以进行分子遗传作图。此外，所有已知的H. villosa对白粉病真菌免疫。Pm21仍然被细胞遗传学定义为一个基因座。本研究采用分子遗传学和细胞遗传学相结合的方法，克隆了一个丝氨酸和苏氨酸蛋白激酶基因Stpk-V。Stpk-V位于Pm21位点。单细胞瞬时表达试验结果表明，Stpk-V能显著降低吸器指数。将Stpk-V基因转化感病小麦品种扬麦158后，获得了与T6 VS. 6AL相似的高抗和广谱抗白粉病的转基因植株。在T6VS.6AL和H. villosa导致它们的易感性增加。Stpk-V能被Bgt和外源H2 O2诱导表达，但在Bgt胁迫下，Stpk-V也能介导内源H2 O2的增加，导致细胞死亡和植物抗性。
Powdery mildew resistance gene Pm21, located on the chromosome 6V short arm of Haynaldia villosa and transferred to wheat as a 6VS.6AL translocation (T6VS.6AL), confers durable and broad-spectrum resistance to wheat powdery mildew. Pm21 has become a key gene resource for powdery mildew resistance breeding all over the world. In China, 12 wheat varieties containing Pm21 have been planted on more than 3.4 million hectares since 2002. Pm21 has been intractable to molecular genetic mapping because the 6VS does not pair and recombine with the 6AS. Moreover, all known accessions of H. villosa are immune to powdery mildew fungus. Pm21 is still defined by cytogenetics as a locus. In the present study, a putative serine and threonine protein kinase gene Stpk-V was cloned and characterized with an integrative strategy of molecular and cytogenetic techniques. Stpk-V is located on the Pm21 locus. The results of a single cell transient expression assay showed that Stpk-V could decrease the haustorium index dramatically. After the Stpk-V was transformed into a susceptible wheat variety Yang-mai158, the characterized transgenic plants showed high and broad-spectrum powdery mildew resistance similar to T6VS.6AL. Silencing of the Stpk-V by virus-induced gene silencing in both T6VS.6AL and H. villosa resulted in their increased susceptibility. Stpk-V could be induced by Bgt and exogenous H2O2, but it also mediated the increase of endogenous H2O2, leading to cell death and plant resistance when the plant was attacked by Bgt.