Electrochemical monitoring of the impact of polymicrobial infections on Pseudomonas aeruginosa and growth dependent medium
Electrochemical monitoring of the impact of polymicrobial infections on Pseudomonas aeruginosa and growth dependent medium
复制标题
DOI：
10.1016/j.bios.2019.111538
复制
发表时间：
2019-10-01
影响因子：
12.6
通讯作者：
Stevenson, Keith J.
中科院分区：
文献类型：
--
作者：
Simoska, Olja;Sans, Marta;Stevenson, Keith J.
机会性人类病原体铜绿假单胞菌（Pa）引起在医疗环境中获得的几种感染。在感染的初始阶段，Pa产生氧化还原活性吩嗪代谢物，包括绿脓菌素（PYO）、5-甲基吩嗪-1-羧酸（5-MCA）和1-羟基吩嗪（OHPHZ），其对周围宿主细胞和/或其他微生物具有毒性作用。这些代谢物的快速和灵敏的检测提供了关于Pa感染发作的重要证据。在此，我们研究了Pa吩嗪生产和动态的差异，在多微生物群落。具体而言，将Pa与两种临床相关的病原体金黄色葡萄球菌（Sa）和大肠杆菌（Ec）共培养，这两种病原体通常分布在Pa感染部位。吩嗪生产率和生物合成动力学电化学监测在48小时内，使用最近开发的透明碳超微电极阵列（T-CUAs）。此外，在两种生长培养基，溶原肉汤（LB）和胰蛋白酶大豆肉汤（TSB）中探索了对吩嗪生产速率和动力学的影响。在两种培养基中的不同多微生物培养物样品中测定PYO和高反应性5-MCA的浓度。结果表明，其他细菌病原体显着影响Pa吩嗪的生产和动力学。特别是，Sa引起TSB中吩嗪产量的减少。然而，在多微生物样品中的Ec的存在下，极大地抑制了LB和TSB中的吩嗪生产速率。最后，介质类型显着影响吩嗪产品的分布，特别是在多微生物共培养，这意味着需要分析标准化的模拟媒体在多微生物群落的研究。
The opportunistic human pathogen Pseudomonas aeruginosa (Pa) causes several infections acquired in a healthcare setting. During initial stages of infection, Pa produces redox-active phenazine metabolites, including pyocyanin (PYO), 5-methylphenazine-1-carboxylic acid (5-MCA), and 1-hydroxyphenazine (OHPHZ), which have toxic effects on surrounding host cells and/or other microbes. Rapid and sensitive detection of these metabolites provides important evidence about the onset of Pa infections. Herein, we investigate differences in Pa phenazine production and dynamics in polymicrobial communities. Specifically, Pa was co-cultured with two pathogens of clinical relevance, Staphylococcus aureus (Sa) and Escherichia coli (Ec), which typically populate infection sites with Pa. Phenazine production rates and biosynthesis dynamics were electrochemically monitored during a 48-h period using recently developed transparent carbon ultramicroelectrode arrays (T-CUAs). Moreover, the effect on phenazine production rates and dynamics was explored in two growth media, lysogeny broth (LB) and tryptic soy broth (TSB). The concentrations of PYO and highly reactive 5-MCA were determined in different polymicrobial culture samples in both media. The results demonstrate that other bacterial pathogens noticeably influence Pa phenazine production and dynamics. In particular, Sa caused a decrease in phenazine production in TSB. However, the presence of Ec in polymicrobial samples drastically inhibited phenazine production rates in both LB and TSB. Conclusively, the media type significantly influences phenazine product distribution, especially in polymicrobial co-cultures, signifying the need for analytical standardization of simulation media in the study of polymicrobial communities.