Small Volume Microrheology to Evaluate Viscoelastic Properties of Nucleic Acid-Based Supra-Assemblies.
Small Volume Microrheology to Evaluate Viscoelastic Properties of Nucleic Acid-Based Supra-Assemblies.
复制标题
用于评估基于核酸的超组装体的粘弹性的小体积微流变学。
DOI：
10.1007/978-1-0716-3417-2_11
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Krasnoslobodtsev,AlexeyV
中科院分区：
文献类型：
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作者：
Gupta,AkhileshKumar;Petersen,Joel;Skelly,Elizabeth;Afonin,KirillA;Krasnoslobodtsev,AlexeyV
颗粒跟踪微流变学是一种表征软物质材料特性的被动微流变学方法。具有产生广泛交联的能力的多组分材料，例如超组装体，可以表现出粘性和弹性性质的复杂相互作用，其中液相的实质性贡献仍然扩散通过系统。微观流变学分析了浸入样品中的微观珠粒的运动，使得评估生物超组装体的流变特性成为可能。这种方法只需要少量的样品和相对简单、廉价的实验装置。本章的目的是描述粒子运动的观察，粒子跟踪的光学设置的校准，成像室的准备，和使用图像分析软件在粘弹性核酸为基础的超组件的粒子跟踪的实验程序。
Particle tracking (PT) microrheology is a passive microrheological approach that characterizes material properties of soft matter. Multicomponent materials with the ability to create extensive crosslinking, such as supra-assemblies, may exhibit a complex interplay of viscous and elastic properties with a substantial contribution of liquid phase still diffusing through the system. Microrheology analyzes the motion of microscopic beads immersed in a sample, making it possible to evaluate the rheological properties of biological supra-assemblies. This method requires only a small volume of the sample and a relatively simple, inexpensive experimental setup. The objective of this chapter is to describe the experimental procedures for the observation of particle motion, calibration of an optical setup for particle tracking, preparation of imaging chambers, and the use of image analysis software for particle tracking in viscoelastic nucleic acid-based supra-assemblies.