Expression of CYP76C6 Facilitates Isoproturon Metabolism and Detoxification in Rice
Expression of CYP76C6 Facilitates Isoproturon Metabolism and Detoxification in Rice
复制标题
CYP76C6 的表达促进水稻异丙隆代谢和解毒
DOI：
10.1021/acs.jafc.1c08137
复制
发表时间：
2022
影响因子：
6.1
通讯作者：
Hong Yang
中科院分区：
文献类型：
--
作者：
Xiao Yan Zhai;Zhao Jie Chen;Jintong Liu;Nan Zhang;Hong Yang
农田和农作物中的农用化学品残留是持续威胁农作物生产、粮食安全和人类健康的严重公共问题之一。了解农作物的腐烂机制对于加速其降解代谢非常重要。在本研究中，在暴露于异丙隆 (IPU) 的水稻中，对编码 CYP76C6 的水稻未表征的细胞色素 P450 基因进行了功能鉴定。为了验证CYP76C6在水稻抵抗IPU毒性中的作用，通过遗传转化和基因编辑技术产生了CYP76C6过表达（OEs）和CRISPR/Cas9敲除突变体水稻。对生长和生理反应的评估表明，OE 系的生长得到改善，IPU 诱导的细胞损伤减弱，IPU 积累受到显着抑制，而 Cas9 系与野生型相比表现出截然不同的表型。 OE系中IPU代谢物和结合物的相对含量均降低，而Cas9系中IPU代谢物和结合物的相对含量增加，表明CYP76C6在IPU降解中发挥关键作用。我们的研究揭示了一种新的调节剂及其在水稻作物中 IPU 腐烂的机制，该机制将在现实中用于降低食品安全和人类健康的环境风险。
Agricultural chemical residues in farmland and crops is one of the serious public issues that constantly threatens crop production, food security, and human health. Understanding their decay mechanism in crops for accelerating their degradative metabolism is important. In this study, a rice uncharacterized cytochrome P450 gene encodingCYP76C6was functionally identified in rice exposed to isoproturon (IPU). To verify the role ofCYP76C6in rice resistance to IPU toxicity,CYP76C6overexpression (OEs) and knockout mutant rice by CRISPR/Cas9 were generated through genetic transformation and gene-editing technologies. Assessment of growth and physiological responses revealed that the growth of OE lines was improved, the IPU-induced cellular damage was attenuated, and IPU accumulation was significantly repressed, whereas the Cas9 lines displayed a contrasting phenotype compared to the wild-type. Both relative contents of IPU metabolites and conjugates in OE lines were reduced and those in Cas9 line were increased, suggesting thatCYP76C6plays a critical role in IPU degradation. Our study unveils a new regulator, together with its mechanism for IPU decay in rice crops, which will be used in reality to reduce environmental risks in food safety and human health.