Surface bioactivation of PEEK by neutral atom beam technology
Surface bioactivation of PEEK by neutral atom beam technology
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DOI：
10.1016/j.bioactmat.2019.02.001
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发表时间：
2019-12-01
影响因子：
18.9
通讯作者：
Smolkov, Alexandre
中科院分区：
文献类型：
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作者：
Khoury, Joseph;Selezneva, Irina;Smolkov, Alexandre
聚醚醚酮（PEEK）是金属植入物的替代品，也是许多应用中的首选材料，包括骨科、脊柱、创伤和牙科。虽然钛（Ti）和钛合金因其生物相容性和骨整合能力而广泛用于许多骨内植入物，但缺点包括导致剪切应力、射线不透性和钛敏感性的刚度。由于PEEK的生物相容性、与骨相似的弹性和射线可透性，许多外科医生更喜欢使用PEEK，然而，由于其惰性，它无法与骨完全结合。加速中性原子束（ANAB）技术已成功用于证明PEEK的体外和体内生物活性增强。在这项研究中，我们进一步表征了经ANAB修饰的PEEK表面，并阐明了暴露于这些表面的牙髓干细胞（DPSC）的附着和遗传效应。ANAB改性导致接触角降低为72.9 +/-4.5度，而对照为92.4 +/-8.5度（p < 0.01），并且平均表面粗糙度降低，但是具有纳米纹理化的表面轮廓。ANAB处理也增加了DPSC附着和增殖的能力，具有相当大的遗传差异，显示出向成骨分化的早期进展。这种表面改性是在不添加涂层或改变PEEK材料的化学成分的情况下实现的。总之，我们表明PEEK表面的ANAB处理增强了植入式医疗器械的生物活性，而无需添加剂或涂层。
Polyetheretherketone (PEEK) is an alternative to metallic implants and a material of choice in many applications, including orthopedic, spinal, trauma, and dental. While titanium (Ti) and Ti-alloys are widely used in many intraosseous implants due to its biocompatibility and ability to osseointegrate, negatives include stiffness which contributes to shear stress, radio-opacity, and Ti-sensitivity. Many surgeons prefer to use PEEK due to its biocompatibility, similar elasticity to bone, and radiolucency, however, due to its inert properties, it fails to fully integrate with bone. Accelerated Neutral Atom Beam (ANAB) technology has been successfully employed to demonstrate enhanced bioactivity of PEEK both in vitro and in vivo. In this study, we further characterize surfaces of PEEK modified by ANAB as well as elucidate attachment and genetic effects of dental pulp stem cells (DPSC) exposed to these surfaces. ANAB modification resulted in decreased contact angle at 72.9 +/- 4.5 degrees as compared to 92.4 +/- 8.5 degrees for control (p < 0.01) and a decreased average surface roughness, however with a nano-textured surface profile. ANAB treatment also increased the ability of DPSC attachment and proliferation with considerable genetic differences showing earlier progression towards osteogenic differentiation. This surface modification is achieved without adding a coating or changing the chemical composition of the PEEK material. Taken together, we show that ANAB processing of PEEK surface enhances the bioactivity of implantable medical devices without an additive or a coating.