The expression of a NMDA receptor gene in guinea-pig myenteric plexus.
The expression of a NMDA receptor gene in guinea-pig myenteric plexus.
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NMDA 受体基因在豚鼠肌间神经丛中的表达。
DOI：
10.1097/00001756-199404000-00030
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发表时间：
1994
期刊：
影响因子：
1.7
通讯作者：
Pleasure,D
中科院分区：
文献类型：
--
作者：
Broussard,DL;Li,X;Pritchett,DB;Lynch,D;Bannermann,PG;Pleasure,D
豚鼠回肠纵肌-肌间神经丛制备物的肌张力研究表明，N-甲基-D-天冬氨酸（NMDA）受体可能存在于肠神经系统中。因此，我们研究了NMDA受体的基因在豚鼠带结肠的表达。使用聚合酶链反应（PCR）扩增基因产物，并使用原位杂交定位其合成。来自肌间神经丛的NMDA受体PCR产物被证明具有与大脑中几乎相同的序列特征。原位杂交研究确定了表达NMDA受体信使RNA的肌间神经元。NMDA受体的遗传表达的证明支持谷氨酸作为肠神经系统中的神经递质的作用。
MUSCLE tension studies of guinea-pig ileum longitudinal muscle-myenteric plexus preparations suggest that the N-methyl-D-aspartate (NMDA) receptor may be present in the enteric nervous system. Therefore, we investigated the expression of a gene for the NMDA receptor in guinea-pig taenia coli. The gene product was amplified using polymerase chain reaction (PCR) and its synthesis localized using in situ hybridization. A NMDA receptor PCR product from the myenteric plexus was demonstrated with nearly identical sequence characteristics to that in the brain. In situ hybridization studies identified myenteric neurons which express NMDA receptor messenger RNA. Demonstration of the genetic expression of the NMDA receptor supports a role for glutamate as a neurotransmitter in the enteric nervous system.