Protein and antibody arrays and their medical applications
Protein and antibody arrays and their medical applications
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DOI：
10.1016/s0022-1759(01)00325-8
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发表时间：
2001-04-01
影响因子：
2.2
通讯作者：
Cahill, DJ
中科院分区：
文献类型：
--
作者：
Cahill, DJ
大规模的基因组学和蛋白质组学策略正在发现许多新的基因产品，现在的挑战是开发高通量的方法来系统地分析这些蛋白质并赋予它们生物学功能。能够获得这些基因产物作为重组表达的蛋白质，将使它们能够被机器人排列来产生蛋白质芯片。其他应用包括使用这些蛋白质产生特定的抗体，这些抗体也可以排列成阵列来生产抗体芯片。这种蛋白质和抗体阵列的可获得性将有助于在一次实验中同时分析数千种相互作用。本章将重点介绍目前用于产生蛋白质和抗体阵列的策略，以及它们在生物研究、医学和诊断中的当前应用。这些方法的缺点，所需的发展，以及蛋白质和抗体阵列的潜在应用将被讨论。(C)2001 Elsevier Science B.V.保留所有权利。
Many new gene products are being discovered by large-scale genomics and proteomics strategies, the challenge is now to develop high throughput approaches to systematically analyse these proteins and to assign a biological function to them. Having access to these gene products as recombinantly expressed proteins, would allow them to be robotically arrayed to generate protein chips. Other applications include using these proteins for the generation of specific antibodies, which can also be arrayed to produce antibody chips. The availability of such protein and antibody arrays would facilitate the simultaneous analysis of thousands of interactions within a single experiment. This chapter will focus on current strategies used to generate protein and antibody arrays and their current applications in biological research, medicine and diagnostics. The shortcomings of these approaches, the developments required, as well as the potential applications of protein and antibody arrays will be discussed. (C) 2001 Elsevier Science B.V. All rights reserved.