Efficient gene targeting by homologous recombination in rice
Efficient gene targeting by homologous recombination in rice
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DOI：
10.1038/nbt737
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发表时间：
2002-10-01
影响因子：
46.9
通讯作者：
Iida, S
中科院分区：
文献类型：
--
作者：
Terada, R;Urawa, H;Iida, S
通过同源重组修饰基因，称为基因打靶，是表征基因功能的最直接方法。然而，在高等植物中，这种方法远非常见的做法。在这里，我们描述了一个有效的和可重复的程序与强大的正/负选择的基因靶向水稻，养活了世界上一半以上的人口，是一个重要的模式植物。约有1%的愈伤组织及其再生的可育植株在目标位点上是杂合的，并且在其基因组中的目标位点上仅发现一个所用选择标记的拷贝。该方法对其他基因的适用性将使获得各种基因靶向水稻品系成为可能。
Modification of genes through homologous recombination, termed gene targeting, is the most direct method to characterize gene function. In higher plants, however, the method is far from a common practice. Here we describe an efficient and reproducible procedure with a strong positive/negative selection for gene targeting in rice, which feeds more than half of the world's population and is an important model plant. About 1% of selected calli and their regenerated fertile plants were heterozygous at the targeted locus, and only one copy of the selective marker used was found at the targeted site in their genomes. The procedure's applicability to other genes will make it feasible to obtain various gene-targeted lines of rice.