Safety assessment of Lactobacillus plantarum JDM1 based on the complete genome.
Safety assessment of Lactobacillus plantarum JDM1 based on the complete genome.
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DOI：
10.1016/j.ijfoodmicro.2011.11.003
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发表时间：
2012-02
影响因子：
5.4
通讯作者：
Zhuo-Yang Zhang;Chang Liu;Yongzhang Zhu;Yan-xia Wei;F. Tian;Guo-Ping Zhao;Xiao-kui Guo
中科院分区：
文献类型：
--
作者：
Zhuo-Yang Zhang;Chang Liu;Yongzhang Zhu;Yan-xia Wei;F. Tian;Guo-Ping Zhao;Xiao-kui Guo
我们基于全基因组序列和相应的表型对益生菌进行了全面的安全性评估。这是在植物乳杆菌JDM 1上进行的，植物乳杆菌JDM 1是在中国广泛使用的商业益生菌菌株。对16种抗生素的最小抑菌浓度（MIC）和JDM 1的生物胺产生进行了测试，以补充传统的口服毒性试验。在JDM 1中总共发现了51个抗生素耐药相关基因、126个毒力相关基因和23个不良代谢相关基因。然而，没有毒素或溶血素编码基因，安全性相关基因很少可转移。这种方法可以推广到提供新的益生菌菌株的深入安全性调查，并大大揭示潜在的危险决定因素及其分子机制。然而，这种分析揭示了理论上的最大风险水平，因为并非所有基因都是有效的，这取决于环境条件。
We performed a comprehensive safety assessment of a probiotic based on the whole genome sequence and corresponding phenotypes. This was performed on Lactobacillus plantarum JDM1, a widely used commercial probiotic strain in China. The minimal inhibitory concentrations (MICs) of sixteen antibiotics and the biogenic amine production of JDM1 were tested to supplement a traditional oral toxicity test. In total, fifty-one antibiotic resistance-associated genes, one hundred twenty-six virulence-associated genes, and twenty-three adverse metabolism-associated genes were found in JDM1. However, there were no toxin or hemolysin encoding genes, and safety-associated genes were rarely transferable. This approach can be generalized to provide a deep safety investigation of novel probiotic strains and greatly reveal the potential danger determinants and their molecular mechanisms. However, this kind of analysis reveals the theoretical maximum risk level as not all genes are efficient depending on environmental conditions.