Differentiation of functional endothelial cells from human induced pluripotent stem cells: A novel, highly efficient and cost effective method
Differentiation of functional endothelial cells from human induced pluripotent stem cells: A novel, highly efficient and cost effective method
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DOI：
10.1016/j.diff.2016.05.004
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发表时间：
2016-10-01
期刊：
影响因子：
2.9
通讯作者：
Zeisberg, Elisabeth M.
中科院分区：
文献类型：
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作者：
Liu, Xiaopeng;Qi, Jing;Zeisberg, Elisabeth M.
人诱导多能干细胞来源的内皮细胞(hiPSC-EC)在研究人类血管发育、体外疾病模型和药物筛选等方面具有重要价值。在这里，我们报道了一种替代的、高效和经济的简单的三步法(中胚层诱导、内皮细胞分化和内皮细胞扩增)将HiPSC直接分化为内皮细胞。我们证明了所述方法在12天内获得CD31+和VE-钙粘附素+双阳性细胞的效率高于80%。最值得注意的是，我们发现HiPSC-EC的分化效率依赖于中胚层分化和内皮细胞分化步骤的优化。(C)2016年国际差异化学会。爱思唯尔出版，版权所有。
Endothelial cells derived from human induced pluripotent stem cells (hiPSC-EC) are of significant value for research on human vascular development, in vitro disease models and drug screening. Here we report an alternative, highly efficient and cost-effective simple three step method (mesoderm induction, endothelial cell differentiation and endothelial cell expansion) to differentiate hiPSC directly into endothelial cells. We demonstrate that efficiency of described method to derive CD31 + and VE-Cadherin+ double positive cells is higher than 80% in 12 days. Most notably we established that hiPSC-EC differentiation efficacy depends on optimization of both mesoderm differentiation and endothelial cell differentiation steps. (C) 2016 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.