In vivo cell recruitment, cytokine release and chemiluminescence response at gold, and thiol functionalized surfaces
In vivo cell recruitment, cytokine release and chemiluminescence response at gold, and thiol functionalized surfaces
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DOI：
10.1016/s0142-9612(99)00115-5
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发表时间：
1999-11-01
期刊：
影响因子：
14
通讯作者：
Thomsen, P
中科院分区：
文献类型：
--
作者：
Källtorp, M;Oblogina, S;Thomsen, P
通过烷基硫醇在金表面的自组装单分子膜技术(SAM)制备了羟基化和甲基化表面。观察种植体表面化学成分对蛋白质沉积和炎性细胞反应的影响。将植入物植入大鼠皮下3h和24h，表面化学性质影响大鼠血浆蛋白的体外吸附(椭圆偏振法/抗体)，少数例外(白蛋白未发现，纤维蛋白原总是存在)。不同的化学处理对细胞的数量和分布(来自种植体和渗出液的DNA)在24 h有影响，但在3 h没有影响，在两个时间段的渗出液中都有HIS48+、ED1+、ED2+和少量的CD5+细胞(流式细胞仪)，细胞的氧化代谢较低，尽管-OH表面的细胞具有最高的佛波酯刺激的化学发光(CL)/DNA。细胞因子IL-1α、IL-1β和TNF-α的水平不受材料表面化学成分的影响。假手术部位的细胞因子浓度/DNA高于植入环境渗出物。这项研究的结果表明，表面化学功能化改变了软组织中种植体周围炎症反应的特定事件。(C)1999爱思唯尔科学有限公司。保留所有权利。
Hydroxylated and methylated surfaces were prepared by the self-assembled monolayer technique (SAM) of alkane thiols on gold. The surfaces were used to evaluate the influence of implant surface chemistry on protein deposition and inflammatory cell response. Implants were inserted subcutaneously in the rat for 3 and 24 h. The surface chemical properties influenced the in vitro rat plasma protein adsorption (ellipsometry/antibody) with few exceptions (albumin not found and fibrinogen always found). The number of recruited cells and their distribution (DNA from implant versus from exudate) was influenced by the different chemistries at 24 h, but not at 3 h. HIS48+, ED1+, ED2+ and small numbers of CD5+ cells were present in the exudate at both time periods (flow cytometry), The cellular oxidative metabolism was low, although cells on -OH surfaces responded with the highest phorbol ester-stimulated chemiluminescence (CL)/DNA. The levels of cytokines IL-1 alpha, IL-1 beta and TNF alpha (ELISA) were not influenced by material surface chemistry. Sham operated sites had a higher cytokine concentration/DNA compared with exudates from an implant milieu. The results of this study show that surface chemical functionalization modifies specific events in the inflammatory response around implants in soft tissues. (C) 1999 Elsevier Science Ltd. All rights reserved.