Control of Lipid Accumulation in the Yeast Yarrowia lipolytica
Control of Lipid Accumulation in the Yeast Yarrowia lipolytica
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DOI：
10.1128/aem.01412-08
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发表时间：
2008-12-01
影响因子：
4.4
通讯作者：
Nicaud, Jean-Marc
中科院分区：
文献类型：
--
作者：
Beopoulos, Athanasios;Mrozova, Zuzana;Nicaud, Jean-Marc
解脂耶氏酵母和酿酒酵母的基因组比较表明，Y.解脂酶的方向是甘油途径。为了将碳流重新导向脂质合成，将编码甘油-3-磷酸脱氢酶异构体的GUT 2基因在Y.解脂素在这项研究中与野生型菌株相比，这种Delta gut 2突变菌株的脂质积累增加了三倍。然而，由于β-氧化，脂质储备的动员发生在退出指数期后。Y.解脂菌含有六种酰基辅酶A氧化酶（Aox），由POX 1至POX 6基因编码，可催化过氧化物酶体β氧化的限制步骤。β gut 2菌株中POX 1至POX 6基因的额外缺失导致脂质含量增加四倍。所有测试菌株的脂质组成均显示高比例的FFA。这些菌株中脂质体的大小和数量取决于脂质组成和积累率。
A genomic comparison of Yarrowia lipolytica and Saccharomyces cerevisiae indicates that the metabolism of Y. lipolytica is oriented toward the glycerol pathway. To redirect carbon flux toward lipid synthesis, the GUT2 gene, which codes for the glycerol-3-phosphate dehydrogenase isomer, was deleted in Y. lipolytica in this study. This Delta gut2 mutant strain demonstrated a threefold increase in lipid accumulation compared to the wild-type strain. However, mobilization of lipid reserves occurred after the exit from the exponential phase due to beta-oxidation. Y. lipolytica contains six acyl-coenzyme A oxidases (Aox), encoded by the POX1 to POX6 genes, that catalyze the limiting step of peroxisomal beta-oxidation. Additional deletion of the POX1 to POX6 genes in the beta gut2 strain led to a fourfold increase in lipid content. The lipid composition of all of the strains tested demonstrated high proportions of FFA. The size and number of the lipid bodies in these strains were shown to be dependent on the lipid composition and accumulation ratio.