11 beta-hydroxysteroid dehydrogenase type 1 promotes differentiation of 3T3-L1 preadipocyte
11 beta-hydroxysteroid dehydrogenase type 1 promotes differentiation of 3T3-L1 preadipocyte
复制标题
11β-羟基类固醇脱氢酶1型促进3T3-L1前脂肪细胞分化
DOI：
10.1111/j.1745-7254.2007.00602.x
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发表时间：
2007-08
期刊：
影响因子：
--
通讯作者：
Liu, Yun
中科院分区：
文献类型：
--
作者：
Xie, Yu;Ding, Guo-xian;Sun, Wen-lan;Sun, Yan;Hu, Gang;Yu, Jing;Zhu, Ting;Liu, Yun
摘要目的：探讨肥胖与2型糖尿病之间的潜在联系11β-羟基类固醇脱氢酶1型（11β-HSD1）与前脂肪细胞分化之间的关系。方法：将小鼠11β-HSD1 siRNA质粒转染至3T3-L1前脂肪细胞（从小鼠胚胎中分离的小鼠Swiss3T3细胞衍生的细胞系）中，检查靶向11β-HSD1的效果。 beta-HSD1 对 3T3-L1 细胞分化的抑制。用 3-异丁基-1-甲黄嘌呤、胰岛素和地塞米松刺激分化。采用实时荧光定量PCR检测基因转录水平。结果：与未转染的3T3-L1细胞相比，转染小鼠11β-HSD1 siRNA的细胞脂质积累明显受到抑制。在用分化诱导试剂刺激之前和刺激之后，在转染细胞中检测到较少的脂滴。转染细胞中脂蛋白脂肪酶和脂肪酸合成酶等脂肪细胞分化相关标志物的表达下调。同样，脂肪细胞分化抑制剂前脂肪细胞因子1的表达在刺激分化后下调，但在转染细胞中没有变化。结论：11β-HSD1可以促进前脂肪细胞分化。基于此，我们提出11β-HSD1可能是肥胖和肥胖诱导的胰岛素抵抗的重要候选介质。
AbstractAim:To investigate the relationship between 11 beta-hydroxysteroid dehydrogenase type 1 (11 beta-HSD1), a potential link between obesity and type 2 diabetes, and preadipocyte differentiation.Methods:Mouse 11 beta-HSD1 siRNA plasmids were transfected into 3T3-L1 preadipocytes (a cell line derived from mouse Swiss3T3 cells that were isolated from mouse embryo), for examination of the effect of targeted 11 beta-HSD1 inhibition on differentiation of 3T3-L1 cells. Differentiation was stimulated with 3-isobutyl-1-methyxanthine, insulin, and dexamethasone. The transcription level of the genes was detected by real-time PCR.Results:Lipid accumulation was significantly inhibited in cells transfected with mouse 11 beta-HSD1 siRNA compared with non-transfected 3T3-L1 cells. Fewer lipid droplets were detected in the transfected cells both prior to stimulation and after stimulation with differentiation-inducing reagents. The expression of adipocyte differentiation-associated markers such as lipoprotein lipase and fatty acid synthetase were downregulated in the transfected cells. Similarly, the expression of preadipocyte factor-1, an inhibitor of adipocyte differentiation, was downregulated upon stimulation of differentiation and had no changes in the transfected cells.Conclusion:11 beta-HSD1 can promote preadipocyte differentiation. Based on this, we propose that 11 beta-HSD1 may be an important candidate mediator of obesity and obesity-induced insulin resistance.
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DOI：
10.1016/s0960-0760(96)00187-2
发表时间：
1997-02
期刊：
The Journal of Steroid Biochemistry and Molecular Biology
影响因子：
--
作者：
Kaiping Yang;M. Khalil;B. Strutt;D. Killinger
通讯作者：
Kaiping Yang;M. Khalil;B. Strutt;D. Killinger
影响因子：
15.9
作者：
Masuzaki, H;Yamamoto, H;Flier, JS
通讯作者：
Flier, JS
影响因子：
15.9
作者：
Masuzaki, H;Yamamoto, H;Flier, JS
通讯作者：
Flier, JS
DOI：
10.1038/oby.2004.3
发表时间：
2004-01-01
期刊：
OBESITY RESEARCH
影响因子：
--
作者：
Engeli, S;Böhnke, J;Sharma, AM
通讯作者：
Sharma, AM
影响因子：
6.7
作者：
FELMER, RICARDO N;CLARK, JOHN A
通讯作者：
CLARK, JOHN A