Split-plot designs for robotic serial dilution assays.
Split-plot designs for robotic serial dilution assays.
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用于机器人连续稀释测定的裂区设计。
DOI：
10.1111/j.1541-0420.2011.01617.x
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发表时间：
2011
期刊：
影响因子：
1.9
通讯作者：
Lansky,DavidM
中科院分区：
文献类型：
--
作者：
Buzas,JeffreyS;Wager,CarrieG;Lansky,DavidM
本文探讨了利用机器人在连续稀释生物测定中有效实施分裂图设计。我们证明了机器人在分块设计下填充板井的最短路径等价于组合学中的最短公超序列问题。我们开发了一种寻找最短公共超序列的算法，提供了一个R实现，并通过模拟探索实现生物测定分裂图设计所需的步骤数分布。我们还展示了如何构建可以用最少的步骤填充的分割图集合，从而证明分割图设计可以用几乎与条带图设计相同的努力实现。最后，我们为从这些设计中得到的数据建模提供了指导方针。
This article explores effective implementation of split-plot designs in serial dilution bioassay using robots. We show that the shortest path for a robot to fill plate wells for a split-plot design is equivalent to the shortest common supersequence problem in combinatorics. We develop an algorithm for finding the shortest common supersequence, provide an R implementation, and explore the distribution of the number of steps required to implement split-plot designs for bioassay through simulation. We also show how to construct collections of split plots that can be filled in a minimal number of steps, thereby demonstrating that split-plot designs can be implemented with nearly the same effort as strip-plot designs. Finally, we provide guidelines for modeling data that result from these designs.