Moving the goal posts: enhancing the sensitivity of PARASHIFT proton magnetic resonance imaging and spectroscopy
Moving the goal posts: enhancing the sensitivity of PARASHIFT proton magnetic resonance imaging and spectroscopy
复制标题
DOI：
10.1039/c3sc51526e
复制
发表时间：
2013-01-01
期刊：
影响因子：
8.4
通讯作者：
Parker, David
中科院分区：
文献类型：
--
作者：
Harvey, Peter;Blamire, Andrew M.;Parker, David
描述了用于化学位移成像的顺磁探针，其中报告叔丁基共振被积分并放置在距镧系元素离子约6至6.5埃处。在这个距离处，共振移动到远离通常的0-12 ppm范围的H-1 NMR光谱窗口的区域，从而允许选择性观察。此外，根据所施加的磁场强度，明智地选择镧系元素离子，导致弛豫速率增强100-200的数量级，允许在光谱学和成像实验中每单位时间更快速的数据采集。增强的灵敏度允许检测这些复合物在小鼠在几分钟内使用移位成像，尾静脉注射剂量的顺序为0.1 mmol kg（-1）。
Paramagnetic probes for chemical shift imaging are described, in which a reporting tert-butyl resonance is integrated and placed about 6 to 6.5 angstrom from a lanthanide ion. At this distance, the resonance is shifted to a region of the H-1 NMR spectral window far away from the usual 0-12 ppm range, permitting selective observation. Furthermore, judicious selection of the lanthanide ion, according to the applied magnetic field strength, leads to relaxation rate enhancements of the order of 100-200, permitting more rapid data acquisition per unit time in both spectroscopy and imaging experiments. The enhanced sensitivity allows the detection of these complexes in mice within a few minutes using shift imaging, following tail vein injection of doses of the order of 0.1 mmol kg(-1).