Maternal light environment interacts with genotype in regulating seed photodormancy in tobacco
Maternal light environment interacts with genotype in regulating seed photodormancy in tobacco
复制标题
母体光环境与基因型相互作用调节烟草种子光休眠
DOI：
10.1016/j.envexpbot.2021.104745
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发表时间：
2022-02-01
影响因子：
5.7
通讯作者：
Li, Zhen Hua
中科院分区：
文献类型：
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作者：
Dong, Shuai;Liu, Yi Ling;Li, Zhen Hua
烟草种子在成熟过程中所经历的环境影响随后的萌发。然而，其分子机制却鲜为人知。本研究旨在揭示母体光环境、基因型及其互作对烟草种子光休眠的影响。结果表明，大多数品种(强光休眠型，暗萌发率小于50%)的新鲜收获烟草种子不能在黑暗中萌发，而少数品种(浅光休眠型，暗萌发率大于50%)在某些环境中成熟的种子可以在不依赖光的情况下萌发，不需要后熟时间。强光休眠种子和浅光休眠种子的转录组比较表明，强光休眠种子在光照和黑暗条件下，光激活植物激素信号相关基因的表达呈现相反的变化。NtPHYB1K326是拟南芥光敏色素B(PHYB)的同源基因，在种子萌发过程中参与光激活的植物激素信号通路。NtPHYB1K326高表达(NtPHYB1K326-OE)种子在黑暗中的萌发频率高于野生型(WT)和RNA干扰(NtPHYB1K326-RNAi)种子。有趣的是，种子在黑暗中的萌发受到光环境的强烈影响。当母株在遮荫环境下成熟时，种子在黑暗中表现出比自然环境下更多的萌发。不同类型的光休眠种子和转NtPHYB1K326基因的种子表现出相似的结果。这些结果表明，种子光休眠可能是一种可遗传的性状，但受到母体植株发育的光环境的强烈影响。
The environment experienced by tobacco seeds during maturation affects subsequent germination. However, its molecular mechanism is poorly understood. This study aimed to reveal the effects of maternal light environment, genotypes, and their interactions on seed photodormancy in tobacco. The results showed that freshly harvested seeds (FS) of majority varieties (strong-photodormant type, dark-germination of FS is less than 50%) in tobacco could not germinate in the dark, while seeds of minority varieties (shallow-photodormant type, dark-germination of FS is more than 50%) that matured in some environments could germinate independent of light and did not require the duration of after-ripening. The transcriptome comparisons of strong-and shallow-photodormant seeds revealed that the expression of genes involved in light-activated plant hormone signals for strong-photodormant seeds showed opposite changes when the seeds were incubated under light and in the dark. NtPHYB1K326, a homologous gene of Arabidopsis thaliana (Arabidopsis) phytochrome B (PHYB) that in the light-activated plant hormone signals pathway during seed germination, was investigated in this study. The germi-nation frequency of NtPHYB1K326 overexpressing (NtPHYB1K326-OE) seeds was higher than that of wild type (WT) and NtPHYB1K326 RNA interference (NtPHYB1K326-RNAi) seeds in the dark. Interestingly, seed germination in the dark was strongly influenced by the light environment. The seeds showed more germination in the dark when mother plants matured under shading environments than under natural environments. Different types of photodormant seeds or NtPHYB1K326-transgenic seeds showed similar results. These findings illustrated that seed photodormancy could be a heritable trait but was strongly influenced by the light environment of maternal plant development.