Allergenicity of recombinant Humulus japonicus pollen allergen 1 after combined exposure to ozone and nitrogen dioxide
Allergenicity of recombinant Humulus japonicus pollen allergen 1 after combined exposure to ozone and nitrogen dioxide
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DOI：
10.1016/j.envpol.2017.11.078
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发表时间：
2018-03-01
影响因子：
8.9
通讯作者：
Wang, Qingyue
中科院分区：
文献类型：
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作者：
Hong, Qiang;Zhou, Shumin;Wang, Qingyue
臭氧（O-3）和二氧化氮（NO2）被认为在加剧空气污染引起的健康问题中起主要作用。然而，联合O-3/NO2对花粉变应原致敏性的影响尚不清楚。葎草花粉过敏原1（Humulus pollene allergene 1，缩写为HJ 1）是一种在东亚引起广泛花粉症的蛋白质。为研究O-3/NO2联合作用对rPj 1致敏性的影响，在原核系统中表达了串联六组氨酸肽标签（His 6）融合的重组rPj 1（rPj 1），并通过His 6亲和层析纯化。用纯化的rBj 1免疫SD大鼠。使用具有高滴度IgG和IgE抗体的大鼠血清进行变应原性定量。将rBj 1暴露于O-3/NO2，并使用免疫的大鼠抗体测定暴露的rBj 1的变应原性的变化。采用串联LC-MS/LC（液相色谱-质谱/液相色谱光谱仪）色谱、紫外光谱和圆二色性（CD）光谱研究了重组蛋白j1的结构变化。我们的数据表明，一个新的二硫键之间的巯基之间的两个相邻的半胱氨酸分子的rbj 1暴露后，连接O-3/NO2，因此IgE结合亲和力增加，过敏性增强。我们的研究结果提供了线索，以阐明背后的机制，空气污染引起的花粉症患病率增加。(C)2017爱思唯尔有限公司版权所有
Ozone (O-3) and nitrogen dioxide (NO2) are thought to play primary roles in aggravating air pollution induced health problems. However, the effects of joint O-3/NO2 on the allergenicity of pollen allergens are unclear. Humulus japonicus pollen allergen 1 (Hum j1) is a profilin protein that causes widespread pollinosis in eastern Asia. In order to study the effects of combined O-3/NO2 on the allergenicity of Hum j1, tandem six-histidine peptide tag (His6)-fused recombinant Hum j1 (rHum j1) was expressed in a prokaryotic system and purified through His6 affinity chromatography. The purified rHum j1 was used to immunize SD rats. Rat sera with high titers of IgG and IgE antibodies against rHum j1 were used for allergenicity quantification. The rHum j1 was exposed to O-3/NO2, and changes in allergenicity of the exposed rHum j1 were assayed using the immunized rat antibodies. Tandem LC-MS/LC (liquid chromatography-mass spectrometer/liquid chromatography spectrometer) chromatography and UV and circular dichroism (CD) spectroscopy were used to study the structural changes in rHum j1. Our data demonstrated that a novel disulfide bond between the sulfhydryl groups of two neighboring cysteine molecules was formed after the rHum j1 exposure to joint O-3/NO2, and therefore IgE-binding affinity was increased and the allergenicity was reinforced. Our results provided clues to elucidate the mechanism behind air pollution-induced increase in pollinosis prevalence. (C) 2017 Elsevier Ltd. All rights reserved.