Circulating osteoblast-lineage cells in humans
Circulating osteoblast-lineage cells in humans
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DOI：
10.1056/nejmoa044264
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发表时间：
2005-05-12
影响因子：
158.5
通讯作者：
Khosla, S
中科院分区：
文献类型：
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作者：
Eghbali-Fatourechi, GZ;Lamsam, J;Khosla, S
背景：虽然目前的证据表明，只有极少数成骨细胞系存在于外周血中，我们假设这些细胞循环，但其浓度被大大低估了，因为使用的检测需要粘附在塑料上。我们进一步推断，这些细胞的浓度在骨形成增加的时期升高，例如在青春期生长。方法：我们使用骨特异性蛋白抗体流式细胞术鉴定11名青少年男性和11名成年男性的循环成骨细胞谱系细胞（平均[+/-SD]年龄，14.5+/-0.7 vs 37.7+/-7.6岁）。采用基因表达和体外、体内成骨实验建立了分选细胞的成骨谱系。结果：在成人外周血中检测到骨钙素阳性细胞和骨特异性碱性磷酸酶阳性细胞（占单个核细胞的1% ~ 2%）。在青春期男孩的血液循环中，骨钙素呈阳性的细胞（他们的骨形成标记物由于青春期的生长而明显增加）是成年受试者的五倍多(P
BACKGROUND:Although current evidence suggests that only a minuscule number of osteoblast-lineage cells are present in peripheral blood, we hypothesized that such cells circulate but that their concentration has been vastly underestimated owing to the use of assays that required adherence to plastic. We further reasoned that the concentration of these cells is elevated during times of increased bone formation, such as during pubertal growth.METHODS:We used flow cytometry with antibodies to bone-specific proteins to identify circulating osteoblast-lineage cells in 11 adolescent males and 11 adult males (mean [+/-SD] age, 14.5+/-0.7 vs. 37.7+/-7.6 years). Gene expression and in vitro and in vivo bone-forming assays were used to establish the osteoblastic lineage of sorted cells.RESULTS:Cells positive for osteocalcin and cells positive for bone-specific alkaline phosphatase were detected in the peripheral blood of adult subjects (1 to 2 percent of mononuclear cells). There were more than five times as many cells positive for osteocalcin in the circulation of adolescent boys (whose markers of bone formation were clearly increased as a result of pubertal growth) as compared with adult subjects (P