Molecularly imprinted polymers prepared using protein-conjugated cleavable monomers followed by site-specific post-imprinting introduction of fluorescent reporter molecules
Molecularly imprinted polymers prepared using protein-conjugated cleavable monomers followed by site-specific post-imprinting introduction of fluorescent reporter molecules
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DOI：
10.1039/c3cc40484f
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发表时间：
2013-01-01
影响因子：
4.9
通讯作者：
Takeuchi, Toshifumi
中科院分区：
文献类型：
--
作者：
Suga, Yusuke;Sunayama, Hirobumi;Takeuchi, Toshifumi
使用蛋白质共轭二硫键可裂解单体制备分子印迹聚合物。在通过二硫键还原去除蛋白质后，将硫醇反应性荧光团引入仅位于印迹腔内部的硫醇残基中，导致将结合事件特异性转导为荧光光谱变化。
Molecularly imprinted polymers were prepared using a protein-conjugated disulfide cleavable monomer. After removing the protein by disulfide reduction, a thiol-reactive fluorophore was introduced into the thiol residue located only inside the imprinted cavity, resulting in specific transduction of the binding events into fluorescence spectral change.