A role for flavin monooxygenase-like enzymes in auxin biosynthesis
A role for flavin monooxygenase-like enzymes in auxin biosynthesis
复制标题
DOI：
10.1126/science.291.5502.306
复制
发表时间：
2001-01-12
期刊：
影响因子：
56.9
通讯作者：
Chory, J
中科院分区：
文献类型：
--
作者：
Zhao, YD;Christensen, SK;Chory, J
尽管人们知道生长素可以调节植物发育的许多过程，并且已经研究了一个多世纪，但植物产生它的机制仍然难以捉摸。在这里，我们报告了拟南芥显性突变体丝兰的特征，该突变体含有升高水平的游离生长素。 YUCCA 编码黄素单加氧酶样酶，属于一个包含至少九个其他同源拟南芥基因的家族，其中一个子集似乎具有冗余功能。色氨酸类似物喂养实验和生化测定的结果表明，YUCCA 催化色胺氨基的羟基化，这是色氨酸依赖性生长素生物合成中的限速步骤。
Although auxin is known to regulate many processes in plant development and has been studied for over a century, the mechanisms whereby plants produce it have remained elusive. Here we report the characterization of a dominant Arabidopsis mutant,yucca, which contains elevated Levels of free auxin. YUCCA encodes a flavin monooxygenase-like enzyme and belongs to a family that includes at Least nine other homologous Arabidopsis genes, a subset of which appears to have redundant functions, Results from tryptophan analog feeding experiments and biochemical assays indicate that YUCCA catalyzes hydroxylation of the amino group of tryptamine, a rate-limiting step in tryptophan-dependent auxin biosynthesis.