Clinical relevance of 1p and 19q deletion for patients with WHO grade 2 and 3 gliomas
Clinical relevance of 1p and 19q deletion for patients with WHO grade 2 and 3 gliomas
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DOI：
10.1007/s11060-008-9563-z
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发表时间：
2008-07-01
影响因子：
3.9
通讯作者：
Hormigo, Adilia
中科院分区：
文献类型：
--
作者：
Iwamoto, Fabio M.;Nicolardi, Linda;Hormigo, Adilia
目的评估胶质瘤中染色体1 p和19 q缺失的频率，并将1 p缺失与2级和3级胶质瘤患者的预后相关，而与组织学亚型无关。方法回顾性分析2000年至2004年间208例WHO 2级和3级胶质瘤患者的1 p/19 q分子研究。从肿瘤组织和生殖系材料中提取DNA，并使用每条染色体的微卫星标记通过PCR进行评估。结果有113名男性和95名女性，中位年龄为40岁。38名患者患有低级别星形细胞瘤（A2），58名患者患有低级别少突胶质细胞瘤（O2），31名患者患有低级别少突星形细胞瘤（OA 2），21名患者患有间变性星形细胞瘤（A3），37名患者患有间变性少突胶质细胞瘤（O3），23名患者患有间变性少突星形细胞瘤（OA 3）。对所有患者进行染色体1 p分析，发现105例患者存在缺失（76%的O2、42%的OA 2、21%的A2、89%的O3、17%的AO 3和14%的A3）。在118例患者中进行了染色体19 q研究，并在46例中显示缺失（56%的O2，45%的OA 2，27%的A2，76%的O3，11%的OA 3和0%的A3）。在多变量分析中，染色体1 p是2级胶质瘤延长PFS（HR = 1.75，P = 0.03）和OS（HR = 3.59，P = 0.02）的预后因素，但不是3级胶质瘤的预后因素（HR = 0.81，P = 0.7，对于PFS; HR = 1.31，P = 0.7，对于OS）。结论染色体1 p缺失是弥漫性2级胶质瘤的一个重要的阳性预后标志，与组织学亚型无关。
Purpose To assess the frequency of chromosomes 1p and 19q deletions in gliomas and to correlate 1p deletion with prognosis in patients with grade 2 and grade 3 gliomas independently of histologic subtype. Methods We retrospectively evaluated 208 patients with WHO grade 2 and 3 gliomas who had 1p/19q molecular studies performed between 2000 and 2004. DNA was extracted from tumor tissue and germline material and evaluated by PCR using microsatellite markers for each chromosome. Results There were 113 men and 95 women with a median age at diagnosis of 40. Thirty-eight patients had a low-grade astrocytoma (A2), 58 low-grade oligodendroglioma (O2), 31 low-grade oligoastrocytoma (OA2), 21 anaplastic astrocytoma (A3), 37 anaplastic oligodendroglioma (O3), and 23 had an anaplastic oligoastrocytoma (OA3). Chromosome 1p analysis was performed in all patients and showed deletions in 105 patients (76% of O2, 42% of OA2, 21% of A2, 89% of O3, 17% of AO3, and 14% of A3). Chromosome 19q studies were performed in 118 patients and showed deletions in 46 (56% of O2, 45% of OA2, 27% of A2, 76% of O3, 11% of OA3 and 0% of A3). On multivariate analyses, chromosome 1p was a prognostic factor for prolonged PFS (HR = 1.75, P = 0.03) and OS (HR = 3.59, P = 0.02) in grade 2 gliomas but not for grade 3 (HR = 0.81, P = 0.7 for PFS; HR = 1.31, P = 0.7 for OS). Conclusion Chromosome 1p deletion is a significant positive prognostic marker in diffuse, grade 2 gliomas regardless of histologic subtype.