Determinants of the microbial community structure of eutrophic, hyporheic river sediments polluted with chlorinated aliphatic hydrocarbons
Determinants of the microbial community structure of eutrophic, hyporheic river sediments polluted with chlorinated aliphatic hydrocarbons
复制标题
DOI：
10.1111/1574-6941.12260
复制
发表时间：
2014-03-01
影响因子：
4.2
通讯作者：
Dejonghe, Winnie
中科院分区：
文献类型：
--
作者：
Hamonts, Kelly;Ryngaert, Annemie;Dejonghe, Winnie
氯代脂肪族烃（CAH）常作为污染地下水基流排入河流.作为生物转化的CAH在受影响的河流沉积物可能是一种有效的修复策略，我们调查了富营养化，CAH污染的Zenne河沉积物的微生物群落结构的决定因素。PCR-DGGE分析结果表明，该河段的细菌、硫酸盐还原菌、地杆菌科、产甲烷古菌和呼吸CAH的Dehalococcoides等微生物群落结构具有较高的多样性;河床深度、沉积物有机碳含量、CAH含量和质地、孔隙水温度和电导率以及甲苯和甲烷浓度对微生物群落结构的变化有显著影响。在一米尺度上，CAH浓度单独解释只有6%的变异Dehalococcoides和硫酸盐还原社区。然而，在厘米尺度上，CAH解释了14.5-35%的变化，在DGGE谱的土壤杆菌科，产甲烷菌，硫酸盐还原菌，细菌，而有机碳含量解释2- 14%。无论是存在的CAH还原脱卤酶基因tceA，bvcA，和vcrA，也不是目标群体的社会结构显着不同的河床位置之间，没有衰减或还原脱氯，表明微生物群落组成是不是一个限制因素，在泽纳沉积物中的生物转化。
Chlorinated aliphatic hydrocarbons (CAHs) often discharge into rivers as contaminated groundwater baseflow. As biotransformation of CAHs in the impacted river sediments might be an effective remediation strategy, we investigated the determinants of the microbial community structure of eutrophic, CAH-polluted sediments of the Zenne River. Based on PCR-DGGE analysis, a high diversity of Bacteria, sulfate-reducing bacteria, Geobacteraceae, methanogenic archaea, and CAH-respiring Dehalococcoides was found. Depth in the riverbed, organic carbon content, CAH content and texture of the sediment, pore water temperature and conductivity, and concentrations of toluene and methane significantly contributed to the variance in the microbial community structure. On a meter scale, CAH concentrations alone explained only 6% of the variance in the Dehalococcoides and sulfate-reducing communities. On a cm-scale, however, CAHs explained 14.5-35% of the variation in DGGE profiles of Geobacteraceae, methanogens, sulfate-reducing bacteria, and Bacteria, while organic carbon content explained 2-14%. Neither the presence of the CAH reductive dehalogenase genes tceA, bvcA, and vcrA, nor the community structure of the targeted groups significantly differed between riverbed locations showing either no attenuation or reductive dechlorination, indicating that the microbial community composition was not a limiting factor for biotransformation in the Zenne sediments.