Brain diphosphoninositide, a new phosphatide having inositol metadiphosphate as a constituent.
Brain diphosphoninositide, a new phosphatide having inositol metadiphosphate as a constituent.
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脑二磷酸肌醇，一种以肌醇偏二磷酸为成分的新型磷脂。
DOI：
10.1016/s0021-9258(18)56993-3
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发表时间：
1949
期刊：
影响因子：
--
通讯作者：
J. Folch
中科院分区：
文献类型：
--
作者：
J. Folch
该实验室的工作已确定脑磷脂 (1, 2) 是三种不同磷脂的混合物；即磷脂酰丝氨酸(3)、磷脂酰乙醇胺和二磷酸肌醇。在初步说明 (4) 中，已表明二磷酸肌醇具有肌醇偏二磷酸酯作为成分。本文包含该陈述的详细证明，并报告了一些其他观察结果，这些观察结果部分确定了二磷酸肌醇的化学结构。选择二磷酸肌醇这个名称是为了强调二磷酸肌醇分子中肌醇偏二磷酸的存在，因为这一特征将该化合物与其他已知的含肌醇磷脂显着区别，其中肌醇和P以单磷酸盐的比例(5-8)存在。粗二磷酸肌醇是通过随附论文(2)中描述的方法从脑磷脂中获得的。该粗制剂含有大量水溶性污染物，其中主要是无机磷酸盐。粗制剂中磷含量约为 11%，经分析证明其中一半是无机磷。它含有大量的钠和钾以及少量的钙和镁。通过透析，可以去除所有的无机磷以及所有的钠和钾。在一个案例中，对粗二磷酸肌醇的可透析级分进行了分析，发现其含有少于 3 个
Work from this laboratory has established that brain cephalin (1, 2) is a mixture of three different phosphatides; namely, phosphatidyl serine (3), phosphatidyl ethanolamine, and diphosphoinositide. In a preliminary note (4) it has been shown that diphosphoinositide has inositol metadiphosphate’as a constituent. The present paper contains detailed proof of this statement and reports some other observations which establish in part the chemical structure of diphosphoinositide. The name diphosphoinositide has been chosen in order to emphasize the presence of inositol metadiphosphate in the diphosphoinositide molecule, since this feature sharply differentiates this compound from other known inositol-containing phosphatides, in which inositol and P are present in the ratio of a monophosphate (5-8).Crude diphosphoinositide is obtained from brain cephalin by a method described in the accompanying paper (2). This crude preparation contains large amounts of water-soluble contaminants, among which inorganic phosphates are predominant. The crude preparation has around 11 per cent phosphorus, half of which proves to be inorganic phosphorus on analysis. It contains large amounts of sodium and potassium and small amounts of calcium and magnesium. By dialysis, it is possible to remove all of the inorganic phosphorus along with all of the sodium and potassium. In one case, in which the dialyzable fraction from the crude diphosphoinositide was analyzed, it was found to contain less than 3 per