Structural messenger RNA contains cytokeratin polymerization and depolymerization signals
Structural messenger RNA contains cytokeratin polymerization and depolymerization signals
复制标题
DOI：
10.1007/s00441-011-1255-x
复制
发表时间：
2011-11-01
影响因子：
3.6
通讯作者：
Major, Francois
中科院分区：
文献类型：
--
作者：
Kloc, Malgorzata;Dallaire, Paul;Major, Francois
我们以前已经证明，Vegt mRNA在非洲爪哇卵母细胞中细胞角蛋白细胞骨架的组织中起着结构性(翻译不依赖)的作用。VEGET基因的缺失导致非洲爪哇卵母细胞营养皮质细胞角蛋白网络的断裂。这种效应可以通过将合成的Vegt RNA注射到卵母细胞中来挽救。在这里，我们证明了在非洲爪哇卵母细胞中Vegt mRNA的结构功能依赖于它的组合信号来诱导或促进细胞角蛋白细丝的解聚和维持细胞角蛋白细丝的解聚状态，并且从整个分子背景中分离出的300个核苷酸的Vegt RNA片段在注射到非洲爪哇卵母细胞时诱导和维持细胞角蛋白细丝的解聚。对三个同源的非洲爪哇蔬菜mRNAs的计算分析表明，在这个300个核苷酸的区域内，存在一个保守的碱基配对(发夹)配置，可能在RNA/蛋白质相互作用中发挥作用。
We have previously shown that VegT mRNA plays a structural (translation-independent) role in the organization of the cytokeratin cytoskeleton in Xenopus oocytes. The depletion of VegT mRNA causes the fragmentation of the cytokeratin network in the vegetal cortex of Xenopus oocytes. This effect can be rescued by the injection of synthetic VegT RNA into the oocyte. Here, we show that the structural function of VegT mRNA in Xenopus oocyte depends on its combinatory signals for the induction or facilitation and for the maintenance of the depolymerization vs. polymerization status of cytokeratin filaments and that the 300-nucleotide fragment of VegT RNA isolated from the context of the entire molecule induces and maintains the depolymerization of cytokeratin filaments when injected into Xenopus oocytes. A computational analysis of three homologous Xenopus VegT mRNAs has revealed the presence, within this 300-nucleotide region, of a conserved base-pairing (hairpin) configuration that might function in RNA/protein interactions.