Programmable Nucleic Acid Nanoswitches for the Rapid, Single-Step Detection of Antibodies in Bodily Fluids
Programmable Nucleic Acid Nanoswitches for the Rapid, Single-Step Detection of Antibodies in Bodily Fluids
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DOI：
10.1021/jacs.7b09347
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发表时间：
2018-01-24
影响因子：
15
通讯作者：
Ricci, Francesco
中科院分区：
文献类型：
--
作者：
Porchetta, Alessandro;Ippodrino, Rudy;Ricci, Francesco
抗体检测在病原体诊断和监测疫苗免疫成功方面发挥着关键作用。然而，当前的血清学技术需要多个耗时的洗涤和孵育步骤，这限制了它们在即时护理 (POC) 诊断和高通量测定中的适用性。我们在此开发了一种核酸纳米开关平台，能够直接直接测量血清和其他体液中的 G 型和 E 型免疫球蛋白（IgG 和 IgE）水平。该系统结合了靶标结合诱导共定位和核酸构象变化纳米开关的优点。由于识别平台的模块化性质，该方法可以潜在地应用于检测抗原可以与核酸链缀合的任何抗体。在这项工作中，我们展示了四种不同抗体的灵敏、快速且经济有效的检测，并证明了这种方法可用于监测接种 AT20 治疗性疫苗的 HIV+ 患者的抗体水平。
Antibody detection plays a pivotal role in the diagnosis of pathogens and monitoring the success of vaccine immunization. However, current serology techniques require multiple, time-consuming washing and incubation steps, which limit their applicability in point-of-care (POC) diagnostics and high-throughput assays. We developed here a nucleic acid nanoswitch platform able to instantaneously measure immunoglobulins of type G and E (IgG and IgE) levels directly in blood serum and other bodily fluids. The system couples the advantages of target binding induced colocalization and nucleic acid conformational-change nanoswitches. Due to the modular nature of the recognition platform, the method can potentially be applied to the detection of any antibody for which an antigen can be conjugated to a nucleic acid strand. In this work we show the sensitive, fast and cost-effective detection of four different antibodies and demonstrate the possible use of this approach for the monitoring of antibody levels in HIV+ patients immunized with AT20 therapeutic vaccine.