Monitoring the nitrification and identifying the endpoint of ammonium oxidation by using a novel system of titrimetry.
Monitoring the nitrification and identifying the endpoint of ammonium oxidation by using a novel system of titrimetry.
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DOI：
10.2166/wst.2011.798
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发表时间：
2011-12
期刊：
影响因子：
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通讯作者：
Xin Zhang;Daijun Zhang;Peili Lu;Cui Bai;PengYing Xiao
中科院分区：
文献类型：
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作者：
Xin Zhang;Daijun Zhang;Peili Lu;Cui Bai;PengYing Xiao
基于我们小组先前开发的混合式呼吸计的结构，开发了一种新的滴定方法，其中两个pH电极安装在测量单元的入口和出口。在LabVIEW中开发了数字滤波和滴定延时校正软件。将滴定仪和呼吸计的硬件和软件集成在一起，构建了一套新的呼吸-滴定系统。应用该系统对一个间歇式硝化过程进行了监测。所得的氧吸收速率（OUR）和氢离子生成速率（HPR）曲线吻合良好，符合生物硝化反应原理。根据OUR和HPR的测量，准确地估计了氧化铵的浓度。此外，HPR测量结果对氨氧化终点的识别灵敏度更高。由于该系统的测量单元完全独立于生物反应器，因此可以用于任何生物反应器中发生的生化反应的在线监测。
Based on the structure of the hybrid respirometer previously developed in our group, a novel implementation for titrimetry was developed, in which two pH electrodes were installed at the inlet and outlet of the measuring cell. The software capable of digital filtering and titration time delay correction was developed in LabVIEW. The hardware and software of the titrimeter and the respirometer were integrated to construct a novel system of respirometry-titrimetry. The system was applied to monitor a batch nitrification process. The obtained profiles of oxygen uptake rate (OUR) and hydrogen ion production rate (HPR) are consistent with each other and agree with the principle of the biological nitrification reaction. According to the OUR and HPR measurements, the oxidized ammonium concentrations were estimated accurately. Furthermore, the endpoint of ammonium oxidation was identified with much higher sensitivity by the HPR measurement. The system could be potentially used for on-line monitoring of biochemical reactions occurring in any kind of bioreactors because its measuring cell is completely independent of the bioreactor.