Nitric oxide inhibition of tobacco catalase and ascorbate peroxidase
Nitric oxide inhibition of tobacco catalase and ascorbate peroxidase
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DOI：
10.1094/mpmi.2000.13.12.1380
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发表时间：
2000-12-01
影响因子：
3.5
通讯作者：
Klessig, DF
中科院分区：
文献类型：
--
作者：
Clark, D;Durner, J;Klessig, DF
我们使用了各种一氧化氮（NO）捐助者证明，NO抑制烟草过氧化氢酶和抗坏血酸过氧化物酶（APX）的活动。这种抑制似乎是可逆的，因为NO供体的去除导致酶活性的显着恢复。与此相反，APX和过氧化氢酶不可逆地抑制过氧亚硝酸盐。NO和过氧亚硝酸盐抑制植物细胞中两种主要的H2O2清除酶的能力表明，NO可能参与病原体攻击后防御反应激活过程中的氧化还原信号。
We used a variety of nitric oxide (NO) donors to demonstrate that NO inhibits the activities of tobacco catalase and ascorbate peroxidase (APX). This inhibition appears to be reversible because removal of the NO donor led to a significant recovery of enzymatic activity. In contrast, APX and catalase were irreversibly inhibited by peroxynitrite. The ability of NO and peroxynitrite to inhibit the two major H2O2-scavenging enzymes in plant cells suggests that NO may participate in redox signaling during the activation of defense responses following pathogen attack.