MOLECULAR ANALYSIS OF THE ALCOHOL DEHYDROGENASE-2 (ADH2) GENE OF MAIZE
MOLECULAR ANALYSIS OF THE ALCOHOL DEHYDROGENASE-2 (ADH2) GENE OF MAIZE
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DOI：
10.1093/nar/13.3.727
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发表时间：
1985-01-01
影响因子：
14.9
通讯作者：
PEACOCK, WJ
中科院分区：
文献类型：
--
作者：
DENNIS, ES;SACHS, MM;PEACOCK, WJ
玉米的Adh2基因具有与玉米Adh1基因密切相关的核苷酸序列，表明这两个基因通过重复事件源自祖基因。编码区在核苷酸水平上保守82%，在氨基酸水平上保守87%。每个基因在相同位置上有 9 个内含子，但它们的核苷酸序列和长度不同。Adh2 编码单个 mRNA，并具有可能的多腺苷酸化信号 AATAAT，位于多腺苷酸化位点上游 15 个碱基处。 Adh2基因与Adh1基因一起由厌氧条件诱导，并且在这些条件下产生增加水平的mRNA。 Adh1 (100 bp) 和Adh2 (126 bp) 转录物的5'非翻译区在序列上存在差异，除了保守区之外，这对于它们的厌氧特异性翻译可能很重要。除了 TATA 框周围的 11 bp 精确同源性和更上游的其他 3 个 8 bp 序列外，两个基因的 3' 和 5' 侧翼序列也存在差异。常见的 8 bp 序列之一 (CACCTCCC) 在两个基因中处于相似的位置（-150 bp 至 -200 bp），可能在基因表达中具有调节功能。
TheAdh2gene of maize has a nucleotide sequence closely related to that of the maizeAdh1gene indicating that the two genes arose from a progenitor gene by a duplication event. The coding regions are 82% conserved at the nucleotide level and 87% conserved at the amino acid level. Each gene has nine introns in identical positions but their nucleotide sequences and lengths differ.Adh2encodes a single mRNA and has a possible polyadenylation signal, AATAAT, fifteen bases upstream from the polyadenylation site. TheAdh2gene together with theAdh1gene is induced by anaerobic conditions and under these conditions produces an increased level of mRNA. The 5′ untranslated regions of the transcripts ofAdh1(100 bp) andAdh2(126 bp) have diverged in sequence except for a conserved region which may be important for their anaerobic-specific translation. The 3′ and 5′ flanking sequences of the two genes are also divergent except for 11 bp of precise homology around the TATA boxes and three other 8 bp sequences further upstream. One of the common 8 bp sequences (CACCTCCC) is in a similar position (−150 bp to −200 bp) in the two genes and may have a regulatory function in gene expression.